我国人类免疫缺陷病毒2型感染的初步回顾性血清学调查

目的]初步了解我国人类免疫缺陷病毒2型（HIV-2）的感染状况。方法]用多种HIV抗体确认方法检测来自全国各地的HIV-2可疑血浆/血清样品,用Genelabs HIV Blot2.2WB检测5份已知HIV-2血浆样品。结果]用Genelabs HIV Blot2.2WB检测54份HIV-2可疑样品,全部呈HIV-1抗体阳性反应且有HIV-2指示带;用HIV-2WB试剂检测,44.4%（24/54）呈HIV-2抗体阳性反应、55.6%（30/54）呈不确定反应;用2种线性免疫试验检测,分别只有3.7%（2/54）和1.9%（1/54）判定为HIV-1/2混合感染,绝大部分仅为HIV-1抗体阳性。对于5份已知HIV-2样品,使用Genelabs HIV Blot2.2WB检测时与HIV-1抗原发生不同程度的交叉反应,但带型明显不同于HIV-1样品。结论]结果提示我国的HIV-2感染很少见,现有的HIV-2WB不宜用于检测HIV-1抗体呈阳性反应的样品。

PRELIMINARY RETROSPECTIVE SEROLOGICAL INVESTIGATION ON HUMAN IMMUNODEFICIENCY VIRUS TYPE 2 INFECTION IN CHINA

Objective]To preliminarily explore the human immunodeficiency virus type 2(HIV-2) infection status in China.Methods]Various confirmatory tests were employed to analyze the serum or plasma samples collected from China and 5 plasma samples with HIV-2 antibody positive were detected using Genelabs HIV Blot 2.2 WB.Results]Among the 54 HIV-2 suspicious samples,all appeared to be HIV-1 antibody positive and HIV-2 antibody indicated using Genelabs HIV Blot 2.2 WB;44.4%(24/54) of samples were HIV-2 antibody positive and 55.6%(30/54) were indeterminate using an HIV-2 WB;only 3.7%(2/54) and 1.9%(1/54) were HIV-1/2 dual infections using two kinds of Line Immuno Assays,respectively,while others were HIV-1 antibody positive only(except 1 was HIV negative using PEPTI-LAV 1-2) .Among the 5 HIV-2 samples detected by Genelabs HIV Blot 2.2 WB,although some of the HIV-1 antibody bands appeared,the reaction patterns were special compared with those for HIV-1 samples.Conclusions]The results indicate that HIV-2 infection is rare in China,and HIV-2 WB is not feasible to detect samples with HIV-1 antibody positive.