夏秋花粉症患者血浆sIgE、类胰蛋白酶、IL-8, IL-12和嗜酸性粒细胞趋化因子水平测定及临床意义

目的:检测夏秋季花粉症(pollinosis)患者血浆类胰蛋白酶(Tryptase), IL-8,IL-12和嗜酸性粒细胞趋化因子(Eotaxin)水平,探讨其临床意义. 方法: 夏秋花粉症患者37例,正常对照23例. 采用双抗体夹心酶联免疫吸附实验(ELISA)检测IL-8, IL-12和嗜酸性粒细胞趋化因子水平, Unicap系统检测血浆类胰蛋白酶. 结果: ①花粉症患者血浆IL-8水平明显高于对照组(t=2.56,P＜0.05);②患者血浆IL-12水平明显低于对照组(P＜0.05);③患者血浆Tryptase和Eotaxin水平与对照组无显著差异(P＞0.05);④患者血浆Tryptase,IL-12和Eotaxin三者之间无相关性(P＞0.05),Tryptase与IL-8也无相关性(P＞0.05),而IL-8与IL-12间存在显著负相关(P＜0.01),IL-8与Eotaxin间存在明显正相关(P＜0.05). 结论: 花粉症的发生与肥大细胞及多种炎症细胞、炎症介质有关,其发病亦与Th1细胞下调机制有关.

Detecion of sIgE, tryptase, IL-8, IL-12, and eotaxin in plasma of patients with pollinosis in summer or autumn and their clinical implications

AIM:To investigate the correlations between plasma levels of tryptase, interleukin-8 (IL-8), interleukin-12 (IL-12) and eotaxin in the patients with grass pollinosis in summer and autumn and their clinical significances. METHODS:A total of 37 patients with grass pollinosis and 23 healthy controls were recruited into the study. Plasma tryptase levels were determined by Unicap Tryptase fluoroimmunoassay. The levels of IL-8, IL-12 and eotaxin were detected by double sandwich ELISA method. RESULTS:The plasma level of IL-8 was significantly increased in the patients with pollinosis as compared with healthy controls (t=2.56, P<0.05). In contrast, the plasma level of IL-12 was significantly decreased in the patients with pollinosis as compared with the healthy controls (P<0.05). But the levels of tryptase and eotaxin had no significant differences between the patients and the volunteers (P>0.05). The significant correlations were only between IL-8 and IL-12 (P<0.01) and between IL-8 and eota-xin (P<0.05). CONLUSION: Occurrence of pollinosis is related to many inflammatory cells including mast cells and inflammatory mediators. The down-regulation of Th1 cytokines may contribute to the pathogenesis of pollinosis.