子宫内膜腺上皮及基质细胞分离、培养作为子宫内膜异位症体外细胞模型的探索

目的 :探索在位子宫内膜腺上皮及基质细胞的分离、培养作为子宫内膜异位症 (EMs)的体外细胞模型。方法 :通过酶解、系列过滤、沉降及贴壁纯化等技术 ,分离、纯化及培养 15份EMs患者的在位子宫内膜腺上皮细胞及其基质细胞 ,并拟传代。结果 :11份标本获得成功 ;每 1g新鲜子宫内膜组织可得到 (8～ 12 )× 10 6个原代基质细胞及 (4～ 8)× 10 6个原代腺上皮细胞 ;基质细胞纯度率可达 95 %以上 ,腺上皮细胞的纯度率约为90 %。基质细胞可以有限的传代 ,腺上皮细胞不能传代。通过改良步骤 ,可提高基质细胞的产量。结论 :在位子宫内膜腺上皮及其基质细胞的分离、培养可作为EMs的体外细胞模型之一。

The exploration of the isolation and culture of eutopic endometrial glandular ep ithelial cell and its stromal cell as an in vitro model of endometriosis.

Objective:To explore the division and culture of eutopic endometrial glandular cells and their stromal cells as the in vitro cell model of endometriois(EMs).Methods:Enzymolysis,serial filtration,sedimentation,and pasted wall purification were used to isolate,purify and culture the endometrial stromal cells in 15 samples of endometrium from patients with EMs.Results: The endometrial cell culture was successfully established in 11 of 15 endometrium samples.From one gramme of fresh endometrial tissue,the yields of primary culture of stromal cells and glandular epithelial cells were about (8～12)×10 6and (4～8)×10 6 respectively.The purities of stromal cells and glandular epithelial cells were 95%and 90% respectively.Stromal cell could be transferred into several passage.However, glandular epithelial cells could not be transferred.The yields of stromal cells could be significantly increased by modification of the procedure. Conclusion:The isolation and culture of endometrial glandular epithelial cells and their stromal cell can be used as one of the in vitro cell models of EMs.