基于多组分含量测定评价氮酮对通络祛痛膏中挥发性药物体外透皮扩散的影响

目的:考察氮酮对通络祛痛膏中挥发性成分体外透皮扩散的影响。方法:采用垂直式Franz扩散池,以离体乳猪腹部皮肤为渗透屏障进行体外透皮扩散实验。使用含20%乙醇的生理盐水溶液作为接受液,转速300 r·min^-1,样品透过液处理方法为转速6000 r·min^-1、离心5 min,残留样品处理方法为加无水乙醇20 mL、超声40 min。以气相色谱仪测定樟脑、薄荷脑、冰片的累积渗透量、膏片残留量、皮肤滞留量,比较氮酮对通络祛痛膏中挥发性药物的体外透皮影响。结果:添加氮酮和不添加氮酮的通络祛痛膏体外透皮特性基本一致,添加氮酮的通络祛痛膏,24 h上述药物累积透过量高于不添加氮酮的通络祛痛膏;皮肤中的滞留量相对升高,膏片残留量相对下降。结论:该试验证明氮酮可作为通络祛痛膏中挥发性药物的的促渗剂,为通络祛痛膏促渗剂的选择提供参考。

Evaluation of the Effect of Azone on Transdermal Diffusion in Vitro of Volatile Drugs in Tongluo Qutong Plaster Based on Multiple Components Determination

Objective: To study the Effect of Azone on Transdermal Diffusion in Vitro of Volatile Drugs in Tongluo Qutong Plaster. Methods: The modified vertical Franz diffusion cell was used to conduct a transdermal experiment in vitro taking the isolated abdominal skin of the Suckling pigs as the transdermal absorption barrier. 20% ethanol saline solution was used as the receiving solution at a speed of 300 r·min^-1. The sample was treated with a speed of 6000 r·min^-1 and centrifugation of 5 min. The residual sample was treated with absolute ethanol (20 mL) and ultrasound (40 min). The cumulative permeability, sample residue and skin retention of camphor, menthol, and borneol were determined by GC to compare the effects of Azone on transdermal delivery in vitro of volatile drugs in Tongluo Qutong Plaster. Results: The in vitro transdermal properties of the two formulations of Tongluo Qutong Plaster were basically the same. The cumulative permeation of the above drugs was higher than that of the Tongluo Qutong Plaster without azone after 24 hours, and the retention of volatile drugs in skin was relatively increased. The residue in the plaster was relatively decreased. Conclusion: This experiment proves that azone can be used as a permeation enhancer of volatile drugs in Tongluo Qutong Plaster, which provides a reference for the selection of permeation enhancers in Tongluo Qutong Plaster.