Generation of long-lived plasma cells to serogroup B Neisseria meningitidis after murine immunisation with an outer membrane protein vaccine |
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Authors: | Cruz Simone C Cruz Aline C Oliveira Juliana M Soares Amanda M Gioia Carolina A C Milagres Lucimar G |
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Affiliation: | Universidade do Estado do Rio de Janeiro, Av. Professor Manoel de Abreu, 444, 3 andar, Departamento de Microbiologia, Imunologia e Parasitologia, CEP: 20550-170, Rio de Janeiro, RJ, Brazil. |
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Abstract: | There is no universal vaccine against serogroup B meningococcus (Men B). We investigated the development of spleen and bone marrow-specific IgG-secreting plasma cells (ASC) in mice immunised with the Cuban outer membrane protein (OMP) vaccine (VA-MENGOC-BC). Bone marrow was the predominant anatomical site of specific ASC and showed constant ASC levels (approximately 4%) at each time point analysed, indicating the production of long-lived ASC. A mean of 2.36 and 0.35% of Men B ASC was detected in spleen after the third dose and 2 months later, respectively, indicating a short-lived population. The data suggest that a short-lived ASC population in spleen was responsible for serum IgG anti-OMP while ASC from bone marrow produced persistent bactericidal antibodies against the vaccine strain. The response to the booster dose was consistent with development of memory B cells by primary vaccination. |
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