兔成骨细胞与纳米氧化锆强韧化高孔隙率磷酸钙人工骨细胞支架生物相容性研究

目的 研究成骨细胞与纳米氧化锆强韧化高孔隙率磷酸钙人工骨细胞支架在体外培养条件下的生物相容性。方法 将成骨细胞株置于含体积分数为10％胎牛血清的DMEM培养基中培养，传代后改用含地塞米松、β-甘油磷酸钠和维生素C的条件培养基培养，分为纳米支架复合细胞组和单纯细胞组，不同时间用倒置相差显微镜、HE染色光镜及扫描电镜观察。MTT法进行细胞增殖测定，并进行细胞微量蛋白含量检测和碱性磷酸酶的定量检测。结果 成骨细胞体外培养时复合或不复合纳米支架均生长良好，表现出典型的细胞形态特征和生物学特性，纳米支架利于细胞的贴附、生长与增殖，并对细胞的功能无不良影响。结论 纳米支架是较理想的骨组织工程支架材料，成骨细胞复合纳米支架用于骨缺损的修复，具有广阔的临床应用前景。

The study on bio-compatibility of rabbit osteoblasts-like cells with hyper-porosity Ca3(PO4)2 artificial bone cells scaffold reinforced by nanometer CrO2 in vitro

Objective To study the bio-compatibility of the rabbit osteoblasts-like cells with hyper-porosity Ca_3(PO4)_2 artificial bone cells scaffold reinforced by nanometer CrO_2 in vitro culture. Methods The osteoblasts-like cells 3T3 - E1 were cultured in becco's modified eagle's medium (DMEM) containing 10% fetal bovine serum. The subsequent cell passaging was conducted in conditioned medium containing dexamethasone, beta-sodium glycerophosphate, and ascorbic acid, with the oste- oblasts in culture then divided into scaffold group (in which the cells were cultured with scaffold) and osteoblasts group (with- out scaffold). The proliferation and differentiation of all the cultured cells were observed at different time points under inverted phase contrast microscope, optical microscope with HE staining and scanning electron microscope respectively, and prolifera- tion of the cultured cells were evaluated by MTT assay. The activity of alkaline phosphatase and total micron-protein contents in these cultured osteoblasts were quantitatively detected. Results The osteoblasts-like cells 3T3-E1 growed well in vitro, regardless of the presence of nanometer scaffold, with biological and morphology characteristics similar to those of normal oste- oblasts. Nanometer scaffold improved the adhesion, growth and proliferation of the cultured cells, showing no adverse effect on the cell functions. Conclusion Nanometer scaffold is an optimal scaffold material for bone tissue engineering, which may po- tentially have clinical application for bone defect repair.