Daxx和NF-κB在儿童急性白血病中的表达及其相关性

目的探讨Daxx在儿童急性白血病(AL)骨髓细胞中的表达及其与NF-κB的相关性，同时观察其与AL临床分型和预后的关系。 方法骨髓涂片取自华中科技大学同济医学院附属同济医院2005—2006年收治的AL患儿和骨髓正常的非恶性血液病患儿,应用免疫组织化学链霉素 亲和素-生物素-过氧化酶复合物(SABC)方法检测50例儿童AL骨髓细胞Daxx和NF-κB的表达，对照组为20例非恶性血液病且骨髓正常患儿。 结果在50例AL患儿中，Daxx和NF-κB蛋白阳性表达率分别为38.0%和56.0%，显著高于正常骨髓组织中的表达(P<0.05)；Daxx与NF-κB表达呈强 相关(P<0.05)；急性淋巴细胞白血病(ALL)患儿高危组Daxx和NF-κB的表达率分别为55.6%和77.8%，标危组分别为0和16.7%，两组之间差异均有 显著性(P<0.05)。 结论Daxx、NF-κB在儿童AL中过度表达，在白血病细胞凋亡和增殖的调控过程中可能起协同作用，且与AL的某些临床特征、疗效和预后密切相 关。

Expression of Daxx in pediatric acute leukemia and its relationship with expression of NF-κB.

AbstractObjectiveTo investigate the expression of Daxx in pediatric acute leukemia(AL) and its relationship with expression of NF-κB and clinical significance. MethodsThe expression of Daxx and NF-κB proteins were detected by immunohistochemical assay in 50 AL children.The control included 20 children with normal bone marrow. ResultsDaxx protein expression rate was 38.0% and NF-κB protein expression rate was 56.0% in 50 AL children.Both of them were significantly higher than that in control group(P<0.05).The expression of Daxx was positively correlated with NF-κB (P<0.05).The expression rates of Daxx and NF-κB were 55.6% and 77.8% respectively in HR ALL,0.0% and 16.7% respectively in SR ALL,and the differences were statistically significant (P<0.05). ConclusionThe over-expression of Daxx and NF-κB may promote tumor genesis and progression in AL.Daxx and NF-κB are also related to some clinical features,treatment outcome and prognostic factors of AL.