脑损伤及胎脑移植的实验研究

目的　观察胎脑细胞悬液延迟移植于脑损伤的生长情况。　方法　46只SD大鼠采用自由落体撞击法制备脑损伤模型。16只仅做单纯脑损伤，伤后14，20，30，40d处死大鼠做HE染色形态学观察；另30只做胎脑移植，于伤后14d做同种胎脑经机械分离的细胞悬液延迟移植，移植后1，5，14，30，75d处死大鼠做HE染色形态学观察。　结果　所有实验大鼠生长良好，无肢体偏瘫。胎脑细胞悬液浓度为4.48×107/ml，活细胞占85.3％，神经细胞占48.2％。伤后14d表现为陈旧性损伤，随时间推移，创伤灶被胶质细胞充填。移植后5d出现免疫细胞，14d胎脑细胞存活，宿主的毛细血管、胶质细胞增生，30d胎脑细胞发生分化，75d胎脑细胞与宿主相互融合。免疫细胞和小部分移植物碎片同时始终存在。　结论　大鼠脑损伤后行胎脑细胞悬液移植，移植物是可以存活并可能发挥功能的。

Experimental study of brain injury and embryonic brain tissue transplantatio n in rats

Objective　To demonstrate morphologic features of the cell suspension of the embryonic brain tissues which were transplanted into lesion c avities of brain injury models. 　Methods　In 46 SD rats, cerebral contusion models were made b y dropping weight method (impact energy: 20 g×30 cm)； of which 16 rats were sa crificed at 14, 20, 30, 40 days after injury and their lesion brains under HE staining, the other 30 rats transplanted by embryonic tissue homogenate at 14 days after injury. The grafted rats were sacrificed at 1, 5, 14, 30, 75 days after implant ation and their brains stained by HE dye. 　Results　All experimental rats survived and no hemiplegia see n. The concentration of fetal brain suspension was 4.48×107 cells/ml. The living cell was 85.3% and neurocyte 48.2%. After 14 days posterior to injury , the main features under microscope was glia proliferation and scar tissue. The immunocytes were found 5 days after transplantation; the grafts survived and t he capillary and the glia proliferation in host were noted 14 days later. Some n eurofibers were derived from cells within the grafts 30 days after transplan tation. The graft was well integrated into host at 75 days posterior to implanta tion. 　Conclusions　The donor cells can be survived and influence ho st and grafts itself.