| 螺内酯对糖尿病大鼠肾小管间质的保护作用 |
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| 引用本文： | 费沛,张建鄂,贾汝汉,涂亚芳,丁国华.螺内酯对糖尿病大鼠肾小管间质的保护作用[J].武汉大学学报(医学版),2009,30(4). |
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| 作者姓名： | 费沛 张建鄂 贾汝汉 涂亚芳 丁国华 |
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| 作者单位： | 1. 郧阳医学院附属太和医院肾内科,湖北,十堰,442000
2. 武汉大学人民医院肾内科,湖北,武汉,430060 |
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| 基金项目： | 湖北省十堰市科技攻关资助项目(编号:2008-04) |
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| 摘 要： | 目的:观察螺内酯对糖尿病大鼠肾小管间质的保护作用并探讨其机制。方法:雄性SD大鼠,随机分为健康对照组、糖尿病组和螺内酯治疗组。8周后检测各组大鼠血压、24 h尿蛋白定量、血肌酐、血钾、血糖、内生肌酐清除率;留取肾组织作PAS染色行病理检查;采用免疫组织化学技术检测近端肾小管上皮细胞醛固酮受体(MR)、检测肾小管-间质中基质金属蛋白酶-2(MMP-2)及α-平滑肌肌动蛋白(-αSMA)表达,并做半定量分析。结果:各组大鼠肾脏近端小管上皮细胞胞质内均有MR表达,与健康对照组相比,糖尿病组近端小管MR表达上调,螺内酯对MR的表达无影响;糖尿病组大鼠肾小管间质MMP-2、-αSMA表达均显著高于对照组(P<0.01),螺内酯治疗8周后,肾组织MMP-2及-αSMA明显下调(P<0.01),肾功能指标及组织病理学损害得以改善,而螺内酯对大鼠的血压、血钾没有影响(P>0.05)。肾组织内-αSMA与MMP-2的表达具有明显的正相关关系(r=0.920,P<0.01)。结论:大鼠近端小管上皮细胞存在MR的表达,糖尿病肾内MR表达增加可能是醛固酮促肾纤维化的部分原因;螺内酯对糖尿病大鼠肾小管间质病变具有保护作用,其机制至少部分与抑制肾小管上皮-肌成纤维细胞转分化,下调肾小管间质中MMP-2、-αSMA表达有关。
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| 关 键 词： | 螺内酯 糖尿病肾病 醛固酮受体 基质金属蛋白酶-2 α-平滑肌肌动蛋白 |
Effects of Spironolactone on the Tubulointerstitium in Progressive Diabetic Kidney Disease |
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| FEI Pei,ZHANG Jiane,JIA Ruhan,TU Yafang,DING Guohua.Effects of Spironolactone on the Tubulointerstitium in Progressive Diabetic Kidney Disease[J].Medical Journal of Wuhan University,2009,30(4). |
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| Authors: | FEI Pei ZHANG Jiane JIA Ruhan TU Yafang DING Guohua |
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| Institution: | FEI Pei1,ZHANG Jian'e1,JIA Ruhan2,TU Yafang2,DING Guohua21Dept.of Nephrology,Taihe Hospital Affiliated to Yunyang Medical College,Shiyan 442000,Hubei,China2Dept.of Nephrology,Renmin Hospital of Wuhan University,Wuhan 430060,China |
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| Abstract: | Objective: To investigate the effects of spironolactone on the tubulointerstitium damage in experimental type 1 diabetic rats.Methods: The SD rats were divided into three groups as follows: normal group,diabetic group,and spironolactone treatment group.Diabetes was induced by intraperitoneal injection of streptozotocin.Eight weeks later,blood pressure was measured in all animals,samples were collected to determine urinary protein excreted in 24 hours,serum creatinine(Scr),serum potassium,blood glucose,and e... |
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| Keywords: | Spironolactone Diabetic Nephropathy Mineralocorticoid Rceptors Matrix Metalloproteinase-2 Alpha Smooth Muscle Actin |
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