FISH技术检测卵巢上皮性肿瘤组织CCND1表达及临床意义

目的:探讨荧光原位杂交技术(FISH)检测卵巢上皮性肿瘤中CCND1基因的表达及其临床意义.方法:应用FISH技术检测45例卵巢正常组织和113例卵巢上皮性肿瘤中CCND1基因的表达.结果:CCND1基因在正常卵巢组织、卵巢良性肿瘤、交界性肿瘤及恶性肿瘤中的扩增表达率分别为11.1％、33.3％、43.5％和66.1％,恶性组的表达扩增率明显高于其他组,差异有统计学意义,P＜0.01；卵巢上皮性癌组织中CCND1基因的表达扩增率与病理分级、临床病理分期及癌组织转移明显相关,差异有统计学意义(P＜0.05),但与年龄、病理学类型无相关性,P＞0.05.结论:CCND1基因的扩增在卵巢肿瘤的发生发展中起重要作用,且与卵巢癌的病理分级、临床分期及癌组织的转移有关,可用以预测肿瘤患者的预后.

Fluorescence in situ hybridization analysis of expression and clinic significance of CCND1 gene in epithelial ovarian neoplasms

OBJECTIVE:To investigation the CCND1 gene and its clinical significance in epithelial ovarian tumors.METHODS:The expression of CCND1 gene in 113 cases of epithelial ovarian tumors and 40 cases of normal ovarian tissue were detected by fluorescence in situ hybridization.RESULTS:Positive expression rates of the CCND1 gene in normal ovarian tissue,benign tumor,borderline tumor and ovarian cancer were separately 11.1%,33.3%,43.5%,66.1%.Compared with other groups,positive expression rate of ovarian cancer was higher(P<0.01).The positive expression rate of CCDN1 gene were significantly related to histological grade,clinicopathological stage and metastasis of ovarian carcinoma(P<0.05),and it was non-significant correlation with the patient’s age and pathological types(P>0.05).CONCLUSIONS:The CCDN1 gene plays a role in the development of ovarian cancer.The positive expression rate of it may serve as a new prognostic maker for ovarian tumor.