LC-MS/MS快速测定大鼠血浆中HHY-002浓度

目的：建立用于测定治疗慢性心律失常药物HHY-002的大鼠血浆测定法。方法：流动相为乙腈-0．1％甲酸（65：35），流速0．200mL／min，色谱柱Phenomenex LunaC18柱（150mm×2．00mm，5μm，5micron），进样量为10μL，柱温35℃，以地西泮为内标。结果：HHY-002在1．98～1013．50ng／mL的范围内呈良好的线性关系（r=0．998），最低定量限达1．98ng／mL，绝对回收率高于80％，日内和日间误差小于10％，方法回收率大于（81．4±4．5）％，符合生物样品分析要求。结论：建立的LC—MS／MS方法专属性强，灵敏度高，可用于HHY-002的体内定量分析。

LC-MS/MS determination of HHY-002 in rat plasma

AIM： A highly sensitive and relia ble LC-MS/MS method was validated for the de termination of HHY-002 in rat plasma, a novel antiarrhythmic. METHODS： After addition of diazepam as internal standard（IS）; LC separa tion with a Phenomenex Luna C18 analytical col umn （150 mm）〈2.00 ram, 5 μm, 5 micron） and quantification by MS/MS after positive ESI. The mobile phase consisted of acetonitrile ： wa ter ： formic acid （65 ： 35 ： 0.1, V/V/V） in an isocratic elution mode pumped at 0. 200 mL/ rain, and with the injection volume of 10μ/L and column oven temperature of 35 ℃. RESULTS：The calibration curve ranging from 1.98 to 1013.50 ng/mL. Lower limit of quantification （LLOQ） was 1.98 ng/mL in 100 μ/L of plasma. Extraction recovery was above 80%, inter and intra day accuracy and precise was below 10%. The method recovery was over（81.4±4.5）%. This method was shown to meet all requirements in bioanalytical determination. CONCLUSION： The method was successfully applied to the de termination of HHY-002 in rat plasma.