干血点采样技术和干血浆采样技术在麦考酚酸药动学分析中的应用

目的探讨干血点采样技术（dried blood spots,DBS）和干血浆采样技术（dried plasma spots,DPS）在麦考酚酸药动学分析中的应用。方法色谱柱为Ecosil C18（150mm×4.6mm,5μm）,流动相为0.01moL/L甲酸水（0.1%甲酸）-乙腈-甲醇（25：45：30）,体积流量为0.4mL/min,柱温为30℃,进样量10μL;质谱检测采用ESI离子源,正离子MRM方式检测;考察可能的影响因素并进行临床研究。结果 DBS法和DPS法方法学和临床实验数据均良好。结论 DBS方法和DPS法较一般处理方法简便,用血量少,储存运输方便,在一些药物的药动学分析中能代替一般的处理方法。

Application of dried blood spots and dried plasma spots technologies in pharmacokinetic analysis of mycophenolic acid

Objective To investigate the application of dried blood spots（DBS） and dried plasma spots（DPS） methods in the pharmacokinetic analysis of mycophenolic acid（MPA）.Methods HPLC was performed on an Ecosil C18 column（150mm×4.6mm,5μm） using 0.1% formic acid（0.01moL/L）-acetonitrile-methanol（25：45：30） as the mobile phase at a flow rate of 0.4mL/min.The column temperature was 30 ℃.Detection was carried out using an ESI interface operating in the multiple reaction monitoring（MRM） mode.Possible factors were studied and clinical trials were carried out.Results The results of DBS and DPS methods and the data of clinical trials were excellent.Conclusion DBS and DPS methods could be alternatives to conventional assays for simple method,wih the advantages of comparable exposure data,small-volume sampling,and easy transportation and preservation.