胎盘生长因子和白细胞介素-6在正常和老化胎盘组织中的表达及其相关性

目的 探讨胎盘生长因子(PLGF)和IL-6在胎盘发育、老化过程中的变化及意义.方法 采用免疫组化法检测正常妊娠早期、中期、晚期胎盘组织及老化胎盘组织中PLGF和IL-6的表达状况.结果 妊娠早、中、晚期及胎盘老化组IL-6的阳性表达率分别为60.O%(6/10)、50.O%(3/6)、42.9%(6/14)及88.9%(8/9).PLGF在妊娠早、中、晚期及老化胎盘组织表达的阳性率分别为60.O%(6/10)、100.0%(6/6)、100.0%(14/14)及55/6%(5/9).PIGF的表达强度妊娠晚期组与早期组(X2=18.89)和胎盘老化组(X2=加.42)比较差异均有统计学意义(P均<0.01);IL-6胎盘老化组与晚期妊娠组比较差异有统计学意义(X2=9.47,P<0.05).妊娠早、中、晚期和胎盘老化组胎盘组织中PLGF与IL-6表达相关性均无统计学意义(X2分别为0.625、0、0和1.41,P均>0.05).结论 胎盘老化时,PLGF的表达较正常晚期妊娠明显减少,提示滋养细胞合成及分泌PIGF的功能下降.胎盘老化时,胎盘中IL-6的表达较正常晚期妊娠组明显增多.提示PLGF和IL-6可能在胎盘老化过程中发挥重要作用.

The expression of placenta growth factor and IL-6 in placentas with different gestational stages and aging placentas

Objective To study the expression of placenta growth factor(PLGF) and IL-6 in placentas with different gestational period.Methods Immunohistochemistry method was used to examine the expression of PLGF and IL-6 in placentas of different normal gestational periods and aging placentas.Results The positive expression rates of IL-6 in the first,second and third trimester of pregnancy were 60.0%(6/10),50/O%(3/6),42.9%(6/14)and 88.9%(8/9),and the positive expression rates of PLGF were 60.0%(6/10),100.O%(6/6),100.O%(14/14)and 55.6%(5/9) respectively.PLGF expression intensity was significantly different among the third trimester of pregnancy placentas,the first trimester of pregnancy placentas (X2=18.89) and the aging placentas (X2=20.42)(P<0.01 for each).IL-6 was significantly different between aging placentas and third trimester of pregnancy placentas(X2=9.47,P<0.05).No statistical significance were found on expression of IL-6 and PLGF among the three trimesters and aging groups (X2=0.625,0,0 and 1.41,respectively,P>0.05).Conclusions The decreasing expression of PLGF in aging placenta tissue illustrates that the PLGF synthesis and secretion is decreased by trophoblast cells but IL-6 expression is remarkably increased,which may play an important role in the pathogenesis of placental senescence.