二甲亚砜对小鼠腹水型网状细胞肉瘤ARS细胞的分化诱导

小鼠ARS腹水型网状细胞肉瘤瘤株加1.5、2.0、2.5%DMSO体外培养,被诱导出现了巨噬细胞样分化。细胞及核缩小,尤以核缩小为著,核质比值下降。2.5%DMSO的作用最显著,核仁缩小仅见于2.5%组。核质比值小于0.5,且核仁亦缩小的细胞近似巨噬细胞。2.5%DMSC使非特异性酯酶阳性细胞增多;Po1ystyrene胶乳颗粒吞噬百分数和吞噬指数亦增高。透射电镜观察,2.5%DMSO处理的、核质比值小于0.5的ARS细胞内,细胞器丰富,Golgi复合体发达,次级溶酶体增多。扫描电镜观察,对照组的大细胞多,表面有多数密集的细长微绒毛。2.5%DMSO处理的ARS,大细胞减少,小细胞增多,微绒毛明显缩短减少。这种小细胞与光镜和透射电镜下观察的小细胞一致,是分化程度较高的巨噬细胞样细胞。2.5%DMSO处理的ARS细胞核分裂指数显著下降。

DMSO INDUCES DIFFERENTIATION OF MURINE ARS ASCITIC RETICULUM SARCOMA CELLS

Murine ARS reticulum sarcoma cells, when incubated with 1.5, 2.0 and 2.5% DMSO in vitro, were induced to differentiate into macrophage like cells. They showed reduction of cellular and nuclear size and lowering of nucleo-cytoplasmic ratio with marked change in nuclear size. The most effective one was 2.5% DMSO which caused marked reduction of nucleolar size. Tumor cells with nucleo-cytoplasmic ratio less than 0.5 and with reduced nucleoli resembled macrophage. 2.5% DMSO induced the increase of non-specific esterase positive cells and the increase of polystyrene phagocytic percentage and phagocytic index. Under EM, 2.5% DMSO treated ARS cells with nucleocytoplasmic ratio less than 0.5 exhibited abundant cytoplasmic organelles, developed Golgi complex and secondary lysosomes. Scanning EM showed that many large cells with numerous dense slender microvilli were observed in the control. Under the action of 2.5% DMSO, large cells were reduced, small cells were increased and microvilli markedly decreased and shortened. These small cells appeared to be differentiated macrophage like cells and were similar to small cells seen under EM and light microscope. In 2.5% DMSO treated groups, the mitotic index of ARS cells were markedly lowered.