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目的探讨单纯性肥胖儿童载脂蛋白(apolipoprotein,Apo)E基因多态性的分布及其对血脂、脂蛋白、载脂蛋白的影响,及其与冠状动脉、心电图改变的关系。并对其早期预测和疾病预防提供理论依据。方法选择2002年12月至2004年12月潍坊医学院附属医院儿科的6～14岁单纯性肥胖儿童89例和健康儿童76例。抽取外周静脉血,测定血清中甘油三酯(TG),总胆固醇(TC),高密度脂蛋白胆固醇(HDLC),低密度脂蛋白胆固醇(LDLC),载脂蛋白A1(ApoA1),载脂蛋白B100(ApoB100)浓度。应用改良的聚合酶链式反应限制性片段长度多态性(PCRRFLP)分析及聚丙烯酰胺凝胶电泳测定儿童ApoE基因型。结果共检出4种ApoE基因型,E3/3、E4/3、E2/3、E4/2,以ε3为最常见。与健康儿童比较,肥胖儿童ε4等位基因频率增高,差异有显著性(P<0.05)。结论单纯性肥胖儿童有ApoE基因多态性的变化,且明显影响小儿血浆脂类代谢,肥胖儿童ApoE4与冠心病有密切相关性。

Clinical studies on the investigation of Apo E polymorphism in children with obesity

??AbstractObjectiveTo investigate the distribution of apolipoprotein(Apo)E polymorphism and association of Apo E genotypes with plasma lipids in children with simple obesity.And to provide a theoretical basis for the prevention of CHD. MethodsFrom Dec.2002 to Dec.2004,89 children with simple obesity and 76 healthy children from 6 to 14 years old were chosen as obesity group and control group.Serum triglyceride(TG)??total cholesterol(TC)??low density lipoprotein cholesterol(LDL??C)??high density lipoprotein cholesterol(HDL??C)??Apolipoprotein A1(Apo A1)??Apolipoprotein B100(Apo B100)were measured after they were fasted for 12 hours.Polymerase chain reaction??restricted fragments length polymorphism(PCR??RFLP)??polyacrylamide gel electrophoresis??and silver staining technique were used for the detection of apolipoprotein E genotype. ResultsFour genotypes of Apo E were detected,and their distribution was imbalanced??most of them being ??3 alleles.Compared with the control group??the frequency of ??4 allele in obesity group was higher(P<0??05).Apo E polymorphisms may affect plasma lipid levels of healthy and obesity children. ConclusionChildren with the simple obesity have different patterns of Apo E polymorphisms as compared with healthy children.Apo E polymorphisms may affect plasma lipids metabolism.