A simple and sensitive chemiluminescence method for the determination of tiopronin for a pharmaceutical formulation

Here we report a rather simple and convenient chemiluminescence (CL) method for the determination of tiopronin. It was based that tiopronin could greatly enhance CL between H₂O₂ and luminol in a basic alkaline solution. Light emission is intense, and even with a simple setup a high sensitivity could be achieved. The linear range was 3 mM–500 nM with a detection limit of 200 nM. Singlet oxygen and hydroxyl radical were suggested to be produced in this reaction and was responsible for the CL of tiopronin. As a preliminary application, this simple method has been successfully applied into the determination of tiopronin in a pharmaceutical formulation.