Comparison of Immobilon-N membrane and other membranes for the detection of HIV-1 genome in high risk patients using PCR |
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| Authors: | Y S Lee M J Collie L K Leung M Seidlin S T Beatrice |
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| Institution: | Division of Retrovirology and Immunobiology, New York City Dept. of Health, New York 10016. |
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| Abstract: | The polymerase chain reaction (PCR) was used for detection of the HIV-1 genome from the peripheral blood lymphocytes of high risk patients. The gag regions of HIV-1 (SK38-SK39) were chosen to amplify viral DNA and the amplified products were spotted onto membrane filters and hybridized with a 32P-labeled SK19 probe. Nitrocellulose, nylon and polyvinylidene difluoride (PVDF) membrane filters were used and compared in dot-blot hybridization. PVDF (Immobilon-N, trade name) filter membranes were demonstrated to be the best membranes on the basis of hybridization data and showed a stronger signal on autoradiograms than the other two types (nitrocellulose and nylon). |
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