先天性巨结肠神经生长因子mRNA表达水平的RT-PCR研究

目的：探讨神经生长因子（ＮＧＦ）的生物作用与先天性巨结肠症发病机制间的关系。方法：采用加入一定量化因素的ＲＴ－ＰＣＲ技术检测了８例先天性巨结肠症、１例神经节细胞过少症以及１例肠神经元发育不良症不同节段肠组织ＮＧＦｍＲＮＡ的表达水平。结果：无神经节细胞的巨结肠狭窄段和节细胞过少的肠组织与扩张段近端正常肠组织相比，其ＮＧＦｍＲＮＡ表达水平降低，阳性率分别是２／９和８／９，肠神经元发育不良的病变肠组织ＮＧＦｍＲＮＡ表达水平亦有下调趋势。结论：ＮＧＦ与肠神经系统发育关系密切，ＮＧＦｍＲＮＡ表达的异常可能参与先天性巨结肠的发病机制；但无节细胞症是多因素综合作用的结果，ＮＧＦ也许仅影响节细胞功能的成熟

RT-PCR Study of Expression of NGF in Hirschsprung''''s Disease

Objective: The aim of the study is to investigate the relationship between the nerve growth factor(NGF)and pathogenesis of Hirschsprung's disease. Methods: The expression of NGF mRNA was studied with RT PCR technique in 8 specimens of Hirschsprung's disease, 1 specimen of hypoganglionosis and 1 specimen of intestinal neuronal dysplasia(IND). Results: The expression of NGF mRNA in aganglionic and hypoganglionic colon(2/9) was less than that in the proximal normal colon(8/9) and normal control. The IND specimen also showed decreased expression. Conclusions: The study suggests that NGF may be involved in development of enteric nerve system. Abnormality in NGF expression may be related to the pathogenesis of Hirschsprung's disease, and affect the maturation of ganglion cells.