Peripheral blood cells chimerism after unrelated cord blood transplantation in children: kinetics,predictive factors and impact on post‐transplant outcome |
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Authors: | Elodie Elkaim Christophe Picard Claire Galambrun Vincent Barlogis Anderson Loundou Catherine Curtillet Claire Oudin Isabelle Thuret Hervé Chambost Gérard Michel |
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Affiliation: | 1. Department of Paediatric Haematology‐Oncology, APHM, La Timone Hospital, Aix‐Marseille University, , Marseille, France;2. établissement Fran?ais du Sang Alpes Méditerranée, , Marseille, France;3. UMR 7268 ADéS, Aix‐Marseille Université/EFS/CNRS, , Marseille, France;4. Department of Public Health ‐EA 3279 Research Unit, University Hospital Marseille, Aix‐Marseille University, , Marseille, France |
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Abstract: | This study aimed to describe kinetics of complete donor chimerism occurrence (cDC, >99·9% donor) after unrelated cord blood transplantation (UCBT), to identify its predictive factors and its impact on post‐transplant outcome. Ninety‐four children who received single UCBT after a myeloablative conditioning regimen had blood chimerism evaluation at predefined post‐transplant dates, using a real‐time polymerase chain reaction method with 0·1% sensitivity. Cumulative incidence of cDC at 1 year post‐transplantation was 61·8%. Three predictive factors were identified in multivariate analysis: history of malignant disease (P = 0·03), older age (above 2·16 years, the first quartile of age, P = 0·0055) and higher level of cord/recipient human leucocyte antigen mismatch (4/6 vs. 5‐6/6, P < 0·001) increased the probability of post‐transplant cDC. Although graft cell dose had a strong impact on haematological recovery, it did not apparently influence cDC occurrence. Early cDC (i.e. more than 99·9% donor chimerism on days 15–30 post‐transplant) appeared useful to predict engraftment (P = 0·003) as well as acute and chronic graft‐versus‐host disease (GvHD). Severe acute or chronic GvHD never occurred in patients with DC ≤99·9%, suggesting than even minimal residual host haematopoiesis is associated with a very low risk of GvHD after UCBT. |
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Keywords: | chimerism unrelated cord blood transplantation childhood human leucocyte antigen compatibilty graft‐versus‐host disease |
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