大鼠生后发育期间胰岛D与A细胞相互关系的形态学观察

①目的观察正常Ｗｉｓｔａｒ大鼠生后发育期间胰岛生长抑素细胞（Ｄ细胞）与胰高血糖素细胞（Ａ细胞）间形态学关系，以探讨两者间功能调节的机理。②方法正常雄性Ｗｉｓｔａｒ大鼠２０只，分为生后７，１８，４５ｄ及成年组，每组５只。常规石蜡包埋，免疫组织化学ＰＡＰ双重染色，分别显示大鼠胰岛Ｄ细胞与Ａ细胞，光镜观察。③结果胰岛Ｄ细胞突起明显，与Ａ细胞形成胞体－胞体、突起－胞体间的紧密接触，并可见Ｄ细胞呈新月形或其长胞突呈半环状及环状，部分或全部包绕Ａ细胞。外分泌部也可见Ｄ细胞与Ａ细胞接触紧密。生后７ｄ与成年组相比Ｄ细胞与Ａ细胞的相邻百分数具有显著性差异（Ｆ＝４．０６，ｑ＝４．７５，Ｐ＜０．０５），其他各组间差异无显著性（ｑ＝０．６５～３．６２，Ｐ＞０．０５）。④结论Ｄ细胞可能通过旁分泌直接调节Ａ细胞的功能活动，这种关系在发育期间不断增强

MORPHOLOGICAL STUDY ON THE RELATIONSHIP BETWEEN PANCREATIC ISLET D CELLS AND A CELLS OF RAT DURING POSTNATAL DEVELOPMENT

Objective To investigate the relationship between pancreatic islet D cells and A cells of rat during postnatal development, and to discuss the possible regulation between these two kinds of cells. Methods Twenty male Wistar rats were evenly divided into 4 groups: 7th day after birth, 18th day after birth, 45th day after birth and adult rats. Pancreatic islet D cells and A cells were displayed with double staining of PAP immunohistochemical technique and observed under microscope. Results D cells had prominent processes, their cell bodies and processes formed close contact with A cells. Some crescent D cells or their ring like processes embraced A cells partially or completely. The close contact between D cells and A cells was also seen in the exocrine region. The contact percentage of D cells with A cells between 7th day after birth and adult group was significantly different( F=4.06,q=4.75,P <0. 05) . Conclusion Pancreatic islet D cells can regulate A cells by means of paracrine and this phenomenon is intensified gradually during postnatal development.