| Isolation and Characterization of Exosomes Derived from Tumor Cells Genetically Expressing Model Antigen |
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| 引用本文： | 修方明,杨云山,蔡志坚,王建莉,曹雪涛. Isolation and Characterization of Exosomes Derived from Tumor Cells Genetically Expressing Model Antigen[J]. 中华微生物学和免疫学杂志(英文版), 2004, 2(4): 278-285 |
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| 作者姓名： | 修方明 杨云山 蔡志坚 王建莉 曹雪涛 |
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| 作者单位： | Institute of Immunology. Zhejiang University，Hangzhou 310031，China，Institute of Immunology. Zhejiang University，Hangzhou 310031，China，Institute of Immunology. Zhejiang University，Hangzhou 310031，China，Institute of Immunology. Zhejiang University，Hangzhou 310031，China，Institute of Immunology. Zhejiang University，Hangzhou 310031，China，Institute of Immunology，Second Military Medical University，Shanghai 200433，China |
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| 摘 要： | Cancer vaccine is an attractive approach for cancer immu-notherapy through activation of specific CTLs. Tradition-ally, cancer vaccines were based on whole autologous orallogeneic cancer cells as well as cancer cell lysates. Butnone of those immunization …
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| 关 键 词： | 肿瘤细胞 外来体 模型抗原 遗传因素 基因表达 |
Isolation and Characterization of Exosomes Derived from Tumor Cells Genetically Expressing Model Antigen |
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| Institute of Immunology. Zhejiang University,Hangzhou ,China. Isolation and Characterization of Exosomes Derived from Tumor Cells Genetically Expressing Model Antigen[J]. Chinese Journal of Microbiology and Immunology, 2004, 2(4): 278-285 |
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| Authors: | Institute of Immunology. Zhejiang University Hangzhou China |
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| Affiliation: | [1]InstituteofImmunology,ZhejiangUniversity,Hangzhou310031,China [2]InstituteofImmunology,SecondMilitaryMedicalUniversity.,Shanghai200433,China |
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| Abstract: | Tumor cell-derived exosomes have been proposed as non-cellular nanomeric vaccine which could induce potent anti- tumor immune response in mice. In order to develop the protocols to prepare tumor cell-derived exosomes for basic research and clinical trail, we isolated exosomes from ovalbumin (OVA)-expressing thymoma cells EG.7-OVA by various preparation methods. We demonstrate the non-sedimentation method is simple, rapid, efficient with higher yield and purity of exosomes. EG.7-OVA-derived exosomes are 40-100 nm in diameter sequestered by lipid bi-layer, and contain rich heat shock protein (HSP) and OVA. The result of the size distribution determination is consistent with the calculation by the visual microscopic inspection, with 90.4% particles at the range of 50-90 nm. Moreover, as a model antigen of the EG.7 cells, OVA concentra- tion in EG.7-derived exosomes can be regarded as a good quality control parameter. Therefore, we have established a platform to efficiently prepare exosomes for tumor immunotherapy. |
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| Keywords: | Exosomes Tumor cells Heat shock proteins Antigen |
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