Cyt-C Bcl-XL和Bcl-XS在大鼠创伤性脑损伤模型中的表达意义

目的 观察大鼠创伤性脑损伤(TBI)后细胞色素C释放及凋亡因子Bcl-XL和Bcl-XS的表达意义.方法 采用大鼠自由落体建立脑损伤模型,分别于伤后2h、6h、12 h、24 h、72 h、120 h、168 h检测大鼠脑挫裂伤侧海马CA2-CA3区细胞色素C染色阳性细胞(免疫组化法),应用Bcl-XL和Bcl-XS原位杂交法检测Bcl-XL和Bcl-XS mRNA表达.结果 脑挫裂伤后,海马CA2-CA3区Cyt-C阳性染色的神经细胞出现具有明显时相性,脑损伤后大鼠脑组织内Bcl-XL、Bcl-XS mRNA出现相应改变.结论 大鼠脑挫裂伤后海马CA2-CA3区细胞有相应变化并出现细胞凋亡.

Expression significance of cytochrome C, Bcl-XL and Bcl-XS after traumatic brain injury in rats

Objective To investigate the regularity of the release of cytochrome C and the expressions of Bcl-XL,Bcl-XS mRNA after traumatic brain injury in rats.Methods Based on the Feeney’s model,the release of cytochrome C in CA2-CA3 region of hippocampus areas of the injured brain was measured by immunohistochemistry.Apoptosis was detected by the Bcl-XL and Bcl-XS in situ hybridization method at 2,6,12,24,48,72,120,168 hours after TBI.Results Using immunohistochemistry method,cytochrome C positive neurons appeared after 1 hour and reached the peak 6 hours later,while fell back since 12 hours,and almost disappeared 24,48,72,120,168 hours later in the CA2-CA3 region of the hippocampus after injury.Using Bcl-XL and Bcl-XS in situ hybridization method,the ratio of Bcl-XL/Bcl-XS was higher after injury.Conclusion The peak of cytochrome C release is earlier than the aoptosis after traumatic brain injury,and cytochrome C is only one of steps in apoptotic cell death after traumatic brain injury.