烧伤大鼠创面再上皮化过程中表皮角质形成细胞基底膜相关基因的表达

目的　检测烧伤大鼠创面再上皮化过程中表皮角质形成细胞(KCs)基底膜(BM)相关基因的表达。　方法　24只SD大鼠造成背部45cm2 深Ⅱ度烫伤, 按照基因表达检测时间随机分为A组(伤后3d)、B组(伤后10d)、C组(伤后14d)、D组(创面完成再上皮化后),每组6只。伤后3、10、14d取距创缘1cm处皮肤标本,创面完成再上皮化后取创面中心皮肤标本,分别制备KCs悬液。另取6只SD大鼠背部皮肤作为正常对照组。利用基因芯片技术检测各组大鼠创面再上皮化不同阶段KCsBM相关基因的差异性表达。　结果　伤后3d,KCs层粘连蛋白γ1、整合素β8基因表达上调,基因表达数据与正常对照比值分别为2. 068和2. 200。再上皮化过程中(伤后10、14d)层粘连蛋白受体1、整合素β1基因表达上调,β2、β7表达下调,基因表达数据与正常对照的比值分别为2. 472、2. 658、0. 419和0. 462。Ⅳ胶型原α1、α3基因各组均上调,基因表达数据与正常对照的比值为2. 547和2 036。　结论　创面再上皮化过程中整合素β1、层粘连蛋白γ1、层粘连蛋白受体1、Ⅳ型胶原α1、α3基因表达上调,有利于新生皮肤BM的构建和KCs与BM之间形成稳定的连接。

Expression of keratinocyte basement membrane related genes during the process of re-epithelialization of burn wound in scalded rats

OBJECTIVE: To study the expression of keratinocyte (KC) basement membrane (BM) related genes during the process of re-epithelialization of burn wound in scalded rats with cDNA microarray technique. METHODS: Twenty-four SD rats were inflicted with deep partial thickness scald with an area of 45 cm(2) on the back, and they were randomly divided into A [3 postscald day (PSD)], B (10 PSD), C (14 PSD) and D (re-epithelialization complete day) groups, with 6 rats in each group. Tissue samples were harvested from 1 cm of wound margin on 3, 10 and 14 PSD. On the re-epithelialization complete day, tissue samples were harvested from the center of the wound in D group and digested with enzyme into KC suspensions. Skin samples from the back of 6 uninjured rats were taken as normal control. The differential expression of KC BM related genes during different stages of re-epithelialization was assayed with cDNA microarray. RESULTS: The expression of laminin (LN) gamma 1 (2.068) and integrin beta8 (2.200) was up-regulated on 3 PSD compared with that in control. The expression of integrin beta1 and LN receptor 1 was up-regulated on 10 and 14 PSD, (2.472 and 2.658), while that of integrin beta2 and beta1 (0.419 and 0.462) down-regulated on 10 and 14 PSD, and the expression of type IV collagen alpha1 and alpha3 was up-regulated during re-epithelialization. CONCLUSION: The experssion of integrin beta1, LN gamma 1, LN receptor 1, type IV collagen alpha1 and alpha3 genes were up-regulated during re-epithelialization, which might be beneficial to the construction of BM in new skin and the formation of stable conjunction between KC and BM.