| 靶向泛素化降解蛋白质技术的应用 |
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| 引用本文： | 马怡晖,张强,陈杰. 靶向泛素化降解蛋白质技术的应用[J]. 中国医学科学院学报, 2010, 32(1). DOI: 10.3881/j.issn.1000-503Ⅹ.2010.01.029 |
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| 作者姓名： | 马怡晖 张强 陈杰 |
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| 作者单位： | 中国医学科学院,北京协和医学院,北京协和医院病理科,北京100730 |
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| 摘 要： | 利用真核细胞内泛素-蛋白酶体途径特异性降解蛋白质原理发展而来的靶向泛素化降解蛋白质技术,可通过合成融合蛋白E3或Protacs小分子,特异性敲减某种选定的蛋白底物.有望成为基因或蛋白功能研究的一种新方法,与DNA/RNA水平的基因沉默技术共同在基础和临床医学研究中发挥重要作用.
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| 关 键 词： | 泛素-蛋白酶体途径 蛋白质降解 泛素化 泛素连接酶 基因沉默 |
Utilization of Targeting Specific Proteins for Ubiquitination and Degradation |
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| MA Yi-hui,ZHANG Qiang,CHEN Jie. Utilization of Targeting Specific Proteins for Ubiquitination and Degradation[J]. Acta Academiae Medicinae Sinicae, 2010, 32(1). DOI: 10.3881/j.issn.1000-503Ⅹ.2010.01.029 |
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| Authors: | MA Yi-hui ZHANG Qiang CHEN Jie |
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| Affiliation: | MA Yi-hui,ZHANG Qiang,CHEN Jie Department of Pathology,PUMC Hospital,CAMS , PUMC,Beijing 100730,China |
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| Abstract: | Ubiquitin-proteasome pathway(UPP) can degradate specific proteins in eukaryotic cells. Targeting specific proteins for ubiquitination and degradation technique,based the theory of UPP and using certain chimeric E3s or protein-targeting chimeric molecules,can specifically knock down the targeted proteins.It is a promising new method for exploring the functions of genes or proteins and,together with gene-silencing technique,may play an important role in basic and clinical research. |
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| Keywords: | ubiquitin-proteasome pathway proteins degradation ubiquitination ubiquitin-ligases gene-silencing |
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