| 坎地沙坦抑制小鼠视网膜新生血管的实验研究 |
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| 引用本文： | 李迅,王嘉俊,刘鹤南,陈晓隆. 坎地沙坦抑制小鼠视网膜新生血管的实验研究[J]. 实用药物与临床, 2011, 14(3) |
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| 作者姓名： | 李迅 王嘉俊 刘鹤南 陈晓隆 |
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| 作者单位： | 1. 中国医科大学附属盛京医院眼科,沈阳,110004
2. 中国医科大学,沈阳,110001 |
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| 基金项目： | 辽宁省自然科学基金资助项目，辽宁省教育厅科研基金资助项目 |
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| 摘 要： | 目的探讨坎地沙坦对小鼠视网膜新生血管(Retinal neovascularization,RNV)的抑制作用。方法采用随机对照实验研究,选取7 d龄C57BL/6J新生小鼠40只,随机分为2组:高氧组和坎地沙坦组,每组20只。坎地沙坦组和高氧组小鼠建立氧诱导视网膜病变模型,出氧箱后,每日分别腹腔注射坎地沙坦40 mg/kg和等量生理盐水,连续5 d。采用荧光素血管灌注视网膜铺片观察视网膜血管形态学改变;制作视网膜组织切片并进行HE染色,计数突破视网膜内界膜的血管内皮细胞核数;采用Western blot检测视网膜血管紧张素Ⅱ(Angio-tensinⅡ,AngⅡ)和血管内皮细胞生长因子(Vascular endothelial growth factor,VEGF)蛋白的表达。结果荧光素血管灌注视网膜铺片:高氧组可见大量新生血管丛,伴明显荧光渗漏,坎地沙坦组较高氧组视网膜新生血管减少,荧光渗漏减轻。突破视网膜内界膜的血管内皮细胞核数:坎地沙坦组较高氧组减少,差异具有统计学意义(P<0.01)。视网膜AngⅡ蛋白表达水平:坎地沙坦组较高氧组下调,差异具有统计学意义(P<0.01)。视网膜VEGF蛋白表达水平:坎地沙坦组较高氧组下调,差异具有统计学意义(P<0.01)。结论早期应用坎地沙坦可一定程度抑制RNV形成。
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| 关 键 词： | 血管紧张素Ⅱ 坎地沙坦 血管内皮生长因子 视网膜新生血管 |
Experimental study of candesartan suppressing retinal neovascularization in mice |
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| LI Xun,WANG Jia-jun,LIU He-nan,CHEN Xiao-long. Experimental study of candesartan suppressing retinal neovascularization in mice[J]. Practical Pharmacy and Clinical Remedies, 2011, 14(3) |
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| Authors: | LI Xun WANG Jia-jun LIU He-nan CHEN Xiao-long |
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| Affiliation: | LI Xun1,WANG Jia-jun2,LIU He-nan1,CHEN Xiao-long1(1.Department of Ophthalmology,Shengjing Hospital of China Medical University,Shenyang 110004,China,2.China Medical University,Shenyang 110001,China) |
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| Abstract: | Objective To investigate the effect of candesartan on suppressing retinal neovascularization(RNV)in mice.Methods Forty seven-day-old C57BL/6J mice were divided into two groups randomly including hyperoxia group and candesartan group,twenty in each group.Candesartan group and hyperoxia group were exposed to 75% oxygen to establish a model of oxygen-induced retinopathy,and received daily intraperitoneal injections of candesartan 40 mg/kg and the equal amount of saline respectively for five days continuously a... |
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| Keywords: | Angiotensin Ⅱ Candesartan Vascular endothelial growth factor Retinal neovascularization |
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