雷公藤红素对人肝癌MHCC97H细胞侵袭转移的影响及机制研究

目的：探讨雷公藤红素对人肝癌MHCC97H细胞侵袭转移的抑制作用及相关机制。方法：应用Transwell技术观察不同浓度（2.5μmol·L^-1、5.0μmol·L^-1、10.0μmol·L^-1）雷公藤红素对MHCC97H细胞侵袭力和迁移力的影响。Westernblot法检测雷公藤红素处理后对C-Jun氨基末端激酶（JNK）信号通路及NF-κB/p65核浆分布变化的影响。结果：雷公藤红素处理后24 h,迁移实验及侵袭实验结果显示,加药组透膜细胞数较对照组明显减少（P〈0.05）。Westerblot结果显示磷酸化JNK表达上调,NF-κB/p65入核减少。结论：雷公藤红素体外具有抑制人肝癌细胞侵袭和转移的作用,其机制可能与活化JNK通路、抑制NF-κBp65入核有关。

Research on the Effect of Celastrol Inhibits Invasion and Metastasis of Human Liver Cancer MHCC97H Cells and Related Mechanism

Objective：To explore the efect of celastrol on the invasion and metastasis of human liver cancer MHCC97H cells and related mechanism. Methods：The transwell chamber was used to investigate the invasion and migration ability of MHCC97H cells after treated with celastrol （2.5,5 and 10 μmol · L^-1 ） for 24 hours. The expression and phosphorylation level of JNK signaling pathway, and subcellular distributions of NF-κB/p65 were all detected through Western blot. Results：After treated with different concentrations of celastrol for 24 h,The membrane-permeating MHCC97H cells in invasion and migration assay were decreased in celastrol treated group comparied with control group. Western blot showed that the expression of p-JNK was increased but total JNK protein were basically unchanged, and expression of NF-κB/p65 decreased in nuclei and increased in the cytoplasm. Conclusion：Celastrol inhibited cell invasion and migration in MHCC97H cells in vitro. The possible mechanism may be related to up-regulation of p-JNK expression, and expression of NF-κB/p65 decreased in nuclei.