A Modified Crossed Radioimmunoelectrophoretic Method for Determination of Allergen-Specific IgG Antibodies |
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Authors: | Ib Søndergaard René Djurup Bent Weeke |
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Institution: | Medical Dept. TA, Division of Allergology and Laboratory for Medical Allergology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark |
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Abstract: | A crossed radioimmunoelectrophoretic (CRIE) method for detection of specific IgG antibodies in patients' sera against horse hair and dander was developed. The unacceptably high non-specific binding encountered when substituting 125I-labelled antihuman IgG for 125I-labelled antihuman IgE in an ordinary CRIE was eliminated by the combined use of 125I-labelled Protein A as detector, and F(ab')2-fragments of the allergen-specific rabbit antibodies. The low background binding thus obtained makes the method useful for detection of specific IgG in sera where the ratio between specific and non-specific IgG is low. Therefore the method should also be applicable to other antigen/allergen systems. |
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Keywords: | IgG antibodies immunotherapy |
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