Scorpions-ARMS法检测非小细胞肺癌患者外周血EGFR基因突变及其临床预测价值的研究

目的应用蝎形扩增阻滞突变系统（Scorpions—ARMS）检测非小细胞肺癌患者外周血EGFR基因突变及其临床预测价值的研究。方法应用蝎型扩增阻滞突变系统（Scorpions—ARMS）检测非小细胞肺癌外周血中EGFR基因第18、19、20及21外显子突变，统计分析EGFR基因突变的相关因素。结果50例非小细胞肺癌患者中，EGFR基因的突变率为30％（15／50）。结果EGFR基因的突变率47．4％（女性）明显高于19．4％（男性）。非吸烟患者EGFR突变率45．5％高于吸烟患者17．9％，差异有统计学意义（P=0．035）。在非小细胞肺癌患者中，腺癌患者EGFR突变率40．6％明显高于非腺癌患者11．1％，差异有统计学意义（P=0．029）。结论Scorpions—ARMS是检测血清游离DNA中EGFR基因突变的可靠有效方法。

Detection EGFR gene mutation in peripheral blood from patients with non-small cell lung cancer by Scorpions-ARMS and its clinical value

Objective To detect EGFR gene mutation in peripheral blood from patients with non-small cell lung cancer by Scorpions-ARMS and its clinical value. Methods Detect the mutations of EGFR gene which contains exons 18, 19, 20 and 21 in 50 cases of peripheral blood by using Scorpions-ARMS. Statistical analysis of EGFR gene mutation related factors. Results EGFR mutations were detected in 15 of 50 （30%） patients with NSCLC. The EGFR mutation rate in females was significantly higher than that of males, 47.4% vs.19.4%, P = 0.035. The EGFR mutation rate in non-smokers was higher than that of smokers, 45.5% vs.17.9%, P=0.029. Conclusion Scorpions-ARMS is reliable and effective for the gaihadetection of EGFR mutations.