胃癌组织中DPC4蛋白的表达及其与临床病理特征的关系

目的探讨胃癌组织中DPC4蛋白的表达及其与临床病理特征的关系。方法采用免疫组化Envision二步法检测50例胃癌及20例癌旁正常组织中DPC4蛋白的表达，并探讨其与患者性别、年龄、肿瘤大小、组织分化程度、浸润深度和淋巴结转移等临床病理特征的关系。结果50例胃癌组织中的DPC4表达阳性31例（62．0％）；20例癌旁正常组织中DPC4表达均为阳性（100．O％）。胃癌组织中DPC4表达的阳性率明显低于癌旁正常组织（X2=10．43，P〈0．01）。胃癌组织中DPC4表达与性别和年龄无明显的统计学关系（P〉0．05）。肿瘤直径〈2cm胃癌DPC4阳性率明显高于肿瘤直径≥2cm，高一中分化胃癌DPC4阳性率明显高于低一未分化，T1-2之期胃癌DPC4阳性率明显高于B3-4期，无淋巴结转移胃癌DPC4阳性率明显高于有淋巴结转移，差异均有统计学意义（P均〈0．05）。结论DPC4基因参与胃癌的发生、发展、浸润和转移过程，其表达程度与胃癌的分化程度、浸润深度和淋巴结转移等临床病理特征密切相关，与胃癌细胞的异常增殖和恶性转化密切相关。

Expression of DPC4 protein in gastric cancer and its relation with clinicopathologic feature

Objective To explore the expression of DPC4 protein in gastric cancer and its relation with clinicopathologicfeature. Methods Detected the expression of DPC4 protein in gastric cancer （50 cases） and normal adjacent tissue （20 cases） by immunohistochemistry envision two step method, and analysis its relation withclinicopathologic feature as. gender, age, size of tumor, histological differentiation degree, infiltration, depth and lymph node metastasis. Results The positive rate of DPC4 in gastric cancer was 62% （31/50）, and 100.0% （20/20） in normal adjacent tissue. It was significantly lower in gastric cancer than that in normal adjacent tissue （χ^2=10.43 ,P 〈 0.01）. The expression of DPC4 showed no significant relation with gender and age （P 〉 0.05）. The positive rate of DPC4 in gastric cancer less than 2 cm was significant higher than that in cancer more than 2 cm. The positive rate of DPC4 in high-middle differentiated cancer was significant higher than that in poorly-undifferentiated cancer. The positive rate of DPC4 in stage T1-2 and none lymph node metastasis were significant higher than that in stage T3-4 and lymph node metastasis respectively （all P 〈 0.05）. Conclusion Gene DPC4, which takes an important part in occurrence, progress, infiltration and metastasis of gastric cancer, has significant relations with biological behavior and prognosis, and its level of expression is closely related with clinicopathologicfeature as histological differentiation degree, infiltration, depth and lymph node metastasis, and is also closely related with dysplasia and vicious transformation.