V8 Nexus高速自动毛细管电泳仪血清蛋白电泳参考区间的建立

目的 初步建立V8 Nexus高速自动毛细管电泳仪(简称V8毛细管电泳仪)血清蛋白电泳(SPE)的参考区间.方法 选取表面健康者649名,按年龄分为18～29岁组、30～39岁组、40～49岁组、50～59岁组及≥60岁组.采用V8毛细管电泳仪[毛细管电泳(CE)法]检测所有对象的血清样本,依据我国卫生行业标准WS/T...

Establishment of reference interval for the detection of serum protein electrophoresis by V8 Nexus high speed automated capillary electrophoresis apparatus

Objective To preliminarily establish a reference interval for serum protein electrophoresis(SPE)by V8 Nexus high speed automated capillary electrophoresis(CE)apparatus.Methods A total of 649 healthy subjects were enrolled in this study and classified into 5 groups according to ages,including 18-29-year-old,30-39-year-old,40-49-year-old,50-59-year-old and≥60-year-old groups.Serum samples were detected by the CE apparatus.The reference interval of CE for SPE detection was evaluated according to the document method of the Health Industry Standard WS/T 402—2012 of the People's Republic of China.Results All data from males and females obeyed normal distribution or approximately normal distribution(P>0.05).There were statistical differences in the levels of albumin,α2 globulin,β1 globulin andγglobulin between males and females(P<0.05),while there was no statistical significance in the results ofα1 globulin,β2 globulin orβglobulin between males and females(P>0.05).In males,there were statistical differences in the levels of albumin,α2 globulin andβ1 globulin between 18-59-year-old groups and≥60-year-old group(P<0.05),but there was no statistical significance in these parameters between 18-59-year-old groups(P>0.05);neither wereα1 globulin,β2 globulin,γglobulin orβglobulin between any groups(P>0.05).In females,there were statistical differences in the levels of albumin,β1 globulin,β2 globulin andβglobulin between 18-59-year-old groups and≥60-year-old group(P<0.05),but there was no statistical significance in these parameters between 18-59-year-old groups(P>0.05);neither wereα1 globulin,α2 globulin orγglobulin between any groups(P>0.05).According to the results of LSD test,SNK test and Z test,the age groups could be combined into one group to establish the reference interval of SPE.Conclusion The reference interval of SPE has been preliminarily established by V8 Nexus high speed automated CE apparatus.Clinical laboratories should establish a reference interval for different sexes or ages according to actual situation.