mCIM与eCIM在产碳青霉烯酶肠杆菌科细菌表型筛查中的价值

目的 分析改良碳青霉烯灭活试验(mCIM)与乙二胺四乙酸碳青霉烯灭活试验(eCIM)对产碳青霉烯酶肠杆菌科细菌表型筛查的效能及应用价值.方法 收集碳青霉烯类耐药肠杆菌科细菌(CRE)117株(实验组)、碳青霉烯类敏感肠杆菌科细菌50株(对照组).分别采用改良Hodge试验(MHT)、mCIM和eCIM进行表型筛查,采用...

Efficacy evaluation and application value of screening carbapenemase in enterobacteriaceae by mCIM and eCIM

Objective To investigate the capacity and application value of modified carbapenem inactivation method(mCIM)and ethylene diamine tetraacetic acid-carbapenem inactivation method(eCIM)for phenotypic detection of carbapenemase-producing Enterobacteriaceae.Methods A total of 117 strains of carbapenem-resistant Enterobacteriaceae(CRE)(experimental group)and 50 strains of carbapenem-sensitive Enterobacteriaceae(control group)were collected.Phenotypic screening was performed by modified Hodge test(MHT),mCIM and eCIM in both groups,respectively.Common carbapenem-resistant genes were detected by polymerase chain reaction(PCR).The consistency of phenotypic screening and gene examinations was analyzed statistically.Results When the detection result of carbapenem-resistant genes was taken as golden standard,the sensitivity of MHT for screening carbapenemase was 87.0%,and the specificity was 100.0%;both sensitivity and specificity of mCIM were 100.0%;the sensitivity and specificity of eCIM were 82.4%and 100.0%respectively.Conclusion mCIM is more sensitive than MHT in screening carbapenemase.The combined detection of mCIM and eCIM can effectively detect carbapenemase and distinguish the type of carbapenemase,which can play a guiding role in clinical medication.