常温肝脏缺血再灌注损伤防治的实验研究

目的和方法：配制一种肝保护液，１００ｍＬ主要含有果糖７５ｇ、谷胱苷肽１５ｇ、别嘌呤醇２５ｇ、维生素Ｅ０３ｇ、地塞米松１０ｍｇ、川芎嗪０４ｇ、山莨菪碱１０ｍｇ和丹参２０ｍＬ。采用大鼠常温肝缺血６０ｍｉｎ－再灌注模型，治疗组４０只大鼠于缺血前１０ｍｉｎ和再灌注前１０ｍｉｎ，静注肝保护液１ｍＬ／ｋｇ，对照组４０只大鼠静注平衡液１ｍｌ／ｋｇ。结果：治疗组术后７ｄ存活率为６０％（１２／２０），而对照组为１５％（３／２０）；治疗组术后血清水平和再灌注后肝坏死范围明显低于对照组（Ｐ＜００５）。结论：肝保护液对大鼠肝常温缺血再灌注损伤具有明显的防治作用，这与减轻肝血窦阻力、增加肝血流，改善肝脏微循环障碍有关。

An experimental study on prevention of liver injury during normothermic ischemia and reperfusion

AIM and METHODS:We made a new solution that contains fructose 7.5g, glutathione 1.5g, allopurinol 2.5g, vitamin E 0.3g,ligustrzin 0.4g, anisodamine 10mg, and Salviamiltionrrhiza 20mL per 100mL. The present study was designed to examine the effect of this new solution. The rat liver was partly liver reperfused after 60min of ischemia. The treatment group( n =40) was injected with the new solution 1mL/kg intravenously 10min before hepatic ischemia and 10min before hepatic reperfusion, respectivety, and the control group ( n =40)received 1mL/kg saline solution RESULTS:In treatment group, 60%(12/20) animals survived 7 days postoperatively, only 15% (3/20) survived in the control group. The improved survival seen in the treated animals was reflected by a reduction in alamine aminotransferase serum levels and attenuate of hepatic necrosis. CONCLUSION: Our new solution attenuate the hepatic injury during mormothermic ischemia/reperfusion, which correlates with decreasing sinusoidal obstruction and increasing liver blood flow. .