左旋精氨酸及硝基左旋精制氨酸对内毒素休克大鼠转归的影响

目的：一氧化氮（Ｎｉｔｒｉｃ Ｏｘｉｄｅ，ＮＯ）在感染性体克中具有多种作用，但其对休克转归的影响目前仍不清楚。本研究用ＮＯ产生的前体物质Ｌ－ａｒｇ和其抑制剂ＮＮＬＡ从正反两个方面探讨其对内毒休克大鼠转归的影响。方法实验大鼠分为生理盐水对照组（ＮＳ）、内毒素（１０ｍｇ／ｋｇ，ｉｐ）组（ＥＴＸ）、内毒素＋ＮＮＬＡ（４ｍｇ／ｋｇ，ｉｐ）组、内毒素＋Ｌ－ａｒｇ（４０ｍｇ／ｋｇ，ｉｐ）组和内毒素＿ＮＮＬＡ＋

Effect of L-arginine and N nitro L-arginine on the outcome in endotoxic shock rat

Objective Nitric oxide(NO)has several pathophysiological effects on septic shock,however,its effect on the outcome of septic shock is still contrasty.This study was to evaluate NO effect on the outcome in endotoxic shock rat by using L arg,the precursor of NO,and NNLA,the inhibitor of NO formation.Methods A total of 213 rats,weighing 180-230g,were subjected to this study. The animals were divided into normal saline control(NS),endotoxin(ETX,LPS 10mg/kg,ip),ETX+NNLA(4mg/kg,ip),ETX+L arg(40mg/kg,ip),and ETX+NNLA+L arg groups.MAP was monitored,24 hour survival rate and the level of plasma NO - 2/NO - 3,the stable metabolic end products of NO,were determined.Results Plasma NO - 2/NO - 3significantly increased and MAP gradually decreased after ETX administration.Although inhibition of NO production with NNLA could significantly increase MAP at early phase,it remarkably decrease 12 and 24 hour survival rate,Administration of L arg could,however,maintain MAP at late phase and significantly improve 24 survival rate.Conclusion NO may have beneficial effect on the outcome of endotoxic shock in the study and inhibition of NO production may be detrimental.