Anti-DNA antibodies in autoimmune disorders by ELISA using nylon as the solid phase |
| |
| Authors: | A Ali R Ali |
| |
| Affiliation: | 1. College of Earth and Environmental Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan;2. Pulmonology Department, Gulab Devi Chest Hospital, Lahore, Pakistan;3. Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan;4. Gomal Centre of Biochemistry and Biotechnology, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan;5. Programmatic Management of Drug-Resistant TB Unit, TB Culture Laboratory, Mufti Mehmood Memorial Teaching Hospital, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan;1. Private Establishment Research Centre of Natural Radioactivity (RCNR), 82, Frunze str., set. Kholmsky, Abinsk Distr., Krasnodar Region, 353302, Russia;2. BitlisEren University, Department of Physics, 13000, Bitlis, Turkey;3. Fırat University, Department of Geological Engineering, 23100, Elazig, Turkey;4. Fırat University, Faculty of Education, 23100, Elazig, Turkey;5. Fırat University, Department of Physics, 23100, Elazig, Turkey;6. Head of Research Department of Hydrogeophysic and Geothermic, Institute of Geophysics, Ivane Javakhishvili Tbilisi State University, 1 Aleksidze Street, Tbilisi, 0171, Georgia;7. Science Faculty, Department of Physics, Suleyman Demirel University, Isparta, Turkey;8. Department of Environmental Studies, University of the Aegean, Mytilene, Greece;9. Laboratory of Hydrogeology, Department of Geology, University of Patras, 26500, Rio, Greece;1. Service de dermatologie, CHU de La Réunion, 97400 Saint-Denis, La Réunion, France;2. Service de dermatologie, CHU de Tours, 37000 Tours, France;3. Université François-Rabelais de Tours, 37000 Tours, France;4. Service de pédiatrie, centre hospitalier de Mamoudzou, 97600 Mamoudzou,, Mayotte, France;5. Service de génétique, CHU de La Réunion, 97400 Saint-Denis, La Réunion, France;1. Regional pharmacovigilance centre, Angers university hospital, 49933 Angers, France;2. Department of internal and vascular medicine, Angers university hospital, 49933 Angers cedex 9, France;1. Department of Pharmacology, Kitasato University School of Medicine, Kanagawa, Japan;2. Department of Endocrinology, Kitasato University School of Medicine, Kanagawa, Japan;3. Department of Surgery, Kitasato University School of Medicine, Kanagawa, Japan;4. Institute of Physiology and Medicine, Jobu University, Gunma, Japan;1. Laboratório de Biofísica Molecular, Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil;2. Laboratório de Cristalografia de Proteínas, Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil;3. Laboratório de Ciências Físicas, Centro de Ciência e Tecnologia, Universidade Estadual do Norte Fluminense Darcy, Campos dos Goytacazes, RJ, Brazil |
| |
| Abstract: | A rapid, simple and highly sensitive enzyme-linked immunosorbent assay (ELISA) utilizing nylon as the solid support is described for the detection of anti-DNA antibodies in autoimmune disorders. The optimal reaction conditions were established with an anti-DNA antibody-positive SLE serum. Fifty-three percent of systemic lupus erythematosus patients, 29 percent of patients with systemic lupus erythematosus with overlapping progressive systemic sclerosis and 10 percent of progressive systemic sclerosis patients were positive for anti-DNA antibodies. The sensitivity of the method was compared with passive hemagglutination and fluorometric assays, the latter using ethidium bromide as intercalating dye. The method described is specific, reproducible and convenient for use in clinical laboratories where large numbers of samples are to be screened for the detection of anti-DNA antibodies. The procedure requires small quantities of antigen or antibody and is, therefore, highly economical. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
