表没食子儿茶素没食子酸酯对中波紫外线诱导人原代表皮黑素细胞光损伤干预作用的研究

目的:观察中波紫外线(UVB)诱导人原代表皮黑素细胞光产物的形成和清除情况,以及表没食子儿茶素没食子酸酯(EGCG)的干预作用.方法:选择10、20、40、80、100 mg/L 5种浓度EGCG作用于体外培养的人表皮黑素细胞72 h,测定细胞增殖活性.采用免疫斑点印迹技术在30 mJ/cm2UVB照射及EGCG干预下.分别取照射后0.5 h和24 h检测环丁烷嘧啶二聚体(CPDs)的产生和清除量.结果:低浓度(＜20 mg/L)EGCG能促进黑素细胞增殖.UVB照射后黑素细胞内光产物形成较快,但清除缓慢.EGCG对UVB诱导光产物的形成无明显影响,但能加速光产物的清除(P＜0.05).结论:EGCG能加速UVB照射后黑素细胞内光产物的清除.

Intervention effects of epigallocatechin-3-gallate on photoproducts induced by UVB in primary human melanocyte

Objective: To investigate the production and clearance of photoproducts induced by UVB irradiation and the intervention effects of epigallocatechin-3-gallate (EGCG) on human primary melanocyte. Methods: The proliferation rates of melanocytes were detected when cells had been incubated with 10, 20, 40, 80 and 100 mg/L EGCG for 72 h, respectively. The production and clearance of cyclobutane pyrimidine dimers (CPDs) were examined by immunodotblot assay at 0.5 and 24 hours after treatment by UVB and EGCG. Results: Low concentration of EGCG (<20 mg/L) promoted the proliferation of cultured human melanocytes. The production of UVB-induced photoproducts was quick, while the clearance was slow. EGCG could facilitate the clearance of photoproducts, but had no effect on the CPDs formation. Conclusion: EGCG can facilitate the clearance of UVB-induced photoproducts in cultured human melanocytes.