外源性神经节苷脂对大鼠脑内注入β-淀粉样蛋白导致的行为损伤的改善作用(英文)

本研究探讨了将β-AP分别注入大鼠海马、隔核和基底巨细胞核后学习和记忆功能的变化。行为测试在β-AP注入 1周后进行。双侧海马 (背部 )注入β-AP后 ,大鼠的分辨学习能力明显下降 ,对照组达到学会标准所需的次数为 2 5± 10 .7;而β-AP组为3 6.7± 9.8,两组差别非常显著 (P<0 .0 0 5 ) .在注射 β-AP后每天给予 40 mg/kg(i.p.)的神经节苷脂 (GAs) ,连续 6d,则大鼠的学习能力可明显改善 ,达到标准所需次数为 2 6.3± 9.2。隔核内注入 β-AP后大鼠的分辨学习能力没有明显的变化 ,而一次性平台测试成绩明显下降 ,同样外源性神经节苷脂可改善这种记忆功能的损伤。本研究还观察了 β-AP注入基底巨细胞核所造成的工作记忆损伤以及 GAs对其的改善作用

EXOGENOUS GANGLIOSIDESIMPROVE THE BEHAVIORAL IMPAIRMENT OF

Effects of intracerebral injection of β-amyloid protein (β-AP) on learning and memory were investigated in rats. The behavioral test was started one week after injections of β-AP. Bilateral injections of β-AP1-40(10 μg/μl for each side) into hippocampus impaired the discrimination learning in tri-equal-arm maze. 25±10.7 trials were needed to reach the criterion, in the control group (n=14), whereas 36.7±9.8 trials were needed in the β-AP treated group(n=14). The difference between them was very significant (P<0.005). When gangliosides [GAs, 40 mg/(kg*d), i.p.] were given for 6 consecutive days after injection of β-AP, the number of trials needed to reach the criterion was reduced significantly (26.3±9.2 trials). After intraseptal injection of β-AP, no significant impairment of discrimination learning was found, but the step-down test revealed the impairment of memory retention. After GAs-treatment, the memory retention was improved obviously. Besides, the deficit of working memory of rats after injection of β-AP into NBM was also investigated, and the ameliorating effect of GAs on impairment of working memory was shown.