颜生胶囊中阿魏酸的定性鉴别与含量测定

 目的 对颜生胶囊中阿魏酸进行定性鉴别与含量测定。方法 采用薄层色谱法鉴别当归、川芎及阿魏酸,采用高效液相色谱法测定阿魏酸含量。色谱条件:色谱柱Kromasi1 5u 100A C18,250 mm×460 mm,流动相:甲醇-1%冰乙酸溶液(30∶70),室温,检测波长320 nm。结果 定性薄层鉴别结果表明,采用硅胶G薄层,以环己烷-二氯甲烷-冰乙酸(16∶16∶2)为展开剂,在365 nm紫外灯下检视,斑点清晰,阴性无干扰;含量测定方法学研究表明,其他成分对阿魏酸测定无干扰,阿魏酸在0.25～1.50 μg/μl浓度范围内,线性关系良好(r=0.9995),精密度良好,对照品溶液及供试品溶液RSD分别为0.35%、0.73%,平均加样回收率99.22%,RSD为1.4%,供试品溶液在6 h内基本稳定。结论 该方法简便、准确、重复性好,可以作为颜生胶囊中当归、川芎的质量控制方法。

Qualitative identification and content determination of ferulic acid in Yansheng capsules

Objective To determine the content of ferulic acid in Yansheng capsules in order to provide reference for pharmaceutical production. Methods TLC was used to identify radix angelicae sinensis and rhizoma ligustici wallichii while high performance liquid chromatography(HPLC)was used for determination of ferulic acid. Chromatographic conditions for ferulic acid included a mobile phase composed of methanol and 1% ice acetic acid solution(30∶70), room temperature, and a detection wavelength of 320 nm. Results The result of TLC showed that the identification spots were clear and negative without any interference observed under a 365 mm UV-lamp using a silican gel layer and with ethane and ice acetic acid (16∶16∶2) as the solvent. Ferulic acid showed a good linearity and precision within the rangeof 0.2515-1.509 μg/μl, r=0.9995. The RSD of the constrast solution and the supplied test-solution was 0.35% and 0.73%, respectively. The average recovery was 99.22%, with an RSD of 1.4%. The supplied test-solution was basically stable within 6 hours. Conclusions This method is simple, accurate and reproducible, which can be used for quality control of Yansheng capsules.