| 猪骨羟基磷灰石对MG63细胞株生物学行为影响的实验研究 |
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| 引用本文： | 王冬赟,刘泉,陈泽涛,陈卓凡.猪骨羟基磷灰石对MG63细胞株生物学行为影响的实验研究[J].临床口腔医学杂志,2012,28(5):265-267. |
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| 作者姓名： | 王冬赟 刘泉 陈泽涛 陈卓凡 |
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| 作者单位： | 中山大学光华口腔医学院 附属口腔医院 广东广州 510055;广东省口腔医学重点实验室 广东广州 510080 |
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| 基金项目： | 国家自然科学基金面上项目（30973355）; 广东省自然科学基金（S2011010004978） |
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| 摘 要： | 目的:检测猪骨羟基磷灰石(Porcine hydroxyapatite,PHA)对MG63细胞株增殖、分化的影响,探讨PHA的体外生物学性能。方法:取猪骨松质用高温烧结的方法制备PHA压片并在其上接种MG63细胞株,分别在3、7、10 d用CCK-8(Cell counting kit-8)实验和碱性磷酸酶(Alkaline phosphatase,ALP)实验检测细胞的增殖及体外矿化能力。结果:相较于阴性对照,PHA组明显促进了MG63细胞的增殖,但对其ALP活性无显著影响。结论:PHA作为一种潜在的骨替代材料,有良好的生物相容性。
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| 关 键 词： | 生物来源羟基磷灰石 MG63细胞株 体外生物学性能 |
Influence of porcine hydroxyapatite on in vitro behavior of MG63 cells |
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| WANG Dong-yun
,
LIU Quan
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CHEN Ze-tao
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CHEN Zhuo-fan.Influence of porcine hydroxyapatite on in vitro behavior of MG63 cells[J].Journal of Clinical Stomatology,2012,28(5):265-267. |
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| Authors: | WANG Dong-yun LIU Quan CHEN Ze-tao CHEN Zhuo-fan |
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| Institution: | 1,2.1.Guanghua School of Stomatology,Hospital of Stomatology,Institute of Stomatological Research,Sun Yat-sen University,Guangdong Guangzhou 510055,China;2.Guangdong Province Key Laboratory of Stomatology,Guangdong Guangzhou 510080,China |
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| Abstract: | Objective：This study was to determine the effect of PHA on the proliferation and ALP activity of MG63 in order to evaluate in vitro biological properties of PHA.Method：PHA was synthesized with chemical and thermal treatment from porcine cancellous bone and compacted to form discs on which MG63 cells were planted.3,7,10 days after planting MG63 cells on PHA discs,CCK-8 assay and ALP activity tests were performed to evaluate the activity of proliferation and in vitro mineralization of cells.Result：Compared to the control,PHA significantly increased the proliferation of MG63 planted on it.Whereas no significant influence on the ALP activity was observed.Conclusion：As a potential bone substitute material,PHA possesses great biocompatibility and bone conductivity. |
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| Keywords: | biological hydroxyapatite MG63 cells in vitro biological property |
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