浙江省羊体内无形体DNA的PCR检测及序列分析

目的了解浙江省羊自然感染无形体的情况。方法在浙江省采集100份羊血标本,应用巢式PCR对羊血标本进行嗜吞噬细胞无形体属16SrRNA基因扩增、测序,并将所测序列与GenBank中注册的基因序列进行相似性比较。结果检出46份阳性,总阳性率为46%,其中山羊的阳性率为48.9%（44/90）,绵羊的阳性率为20.0%（2/10）;测序后与GenBank中注册序列比对分析,41份山羊和2份绵羊血标本与牛无形体相同基因区接近,3份山羊血标本与嗜吞噬细胞无形体相同基因区接近;不同地区的羊均感染羊无形体,不同地区感染率差异有统计学意义（χ2=15.36,P〈0.01）。结论该地区家畜动物中存在较高的牛无形体的自然感染,且山羊为嗜吞噬细胞无形体储存宿主之一,该地区是人粒细胞无形体病的潜在疫源地。

Detection and sequence analysis of Anaplasma DNA in goats and sheep from Zhejiang province

Objective To explore the prevalence of Anaplasma natural infection in goats and sheep in Zhejiang province.Methods A nested polymerase chain reaction（PCR） method was used to amplify the fragments of Anaplasma 16SrDNA in the blood specimens from goats and sheep.The positive PCR products were sequenced.The sequences of 16S rRNA gene fragments from positive samples were compared with Anaplasma sequences deposited in GenBank.Results Among the 100 goats and sheep,46（46%） showed positive,in which the positive rate was 48.9%（44/90） in goats and 20.0%（2/10） in sheep,respectively.By sequence comparison,16S rDNA fragments in 41 goats and 2 sheep were closed to those of Anaplasma bovis,and 16S rDNA fragments in 3 sheep were closed to those of Anaplasma phagocytophilum.Anaplasma infections were found in goats and sheep in different regions of Zhejiang province.There was significant difference in infection rate in these regions（χ2=15.36,P0.01）.Conclusion Anaplasma infections exist in goats and sheep in Zhejiang province.Goats might serve as the important host for Anaplasma phagocytophilum,suggesting the potential risk for the domestic animals and human beings in this area.Further investigation is still needed.