铁皮石斛及金钗石斛菌根真菌的分离及其生物活性测定

目的 :对我国云南、四川等地采集的野生铁皮石斛Dendrobiumcandidum和金钗石斛D .nobile根中的内生真菌进行分离并测定其生物活性。方法 :采用平板分离法分离内生真菌 ;所获得真菌分别与铁皮石斛种子、铁皮石斛及金钗石斛无菌苗共培养测定其生物活性。结果 :分离获得内生真菌 2 5种 ,主要属于担子菌Basidiomy cotina和半知菌Deuteromycotina;生物活性测定结果表明 ,其中 5种真菌可促进铁皮石斛种子萌发 ;7种真菌可与铁皮石斛和金钗石斛幼苗形成共生关系 ,但仅有 3种真菌对幼菌有促生作用。结论 :分离和筛选促进石斛生长发育的适宜菌根真菌 ,是菌根技术应用于石斛生产的关键问题之一。

Isolation and Biological Activity of Mycorrhizal Fungi from Dendrobium candidum and D.nobile

Objective:To isolate the mycorrhizal fungi from the roots of wild Dendribium candidum and D. nobile collected from Yunnan and Sichuan provinces and determine their biological activities.Method:The isolation was completed using solidified potato dextrose agar (PDA, Difco) of plates and the biological activities were tested by means of symbiotic germination of fungus seedlings. Results:25 Species of mycorrhizal fungi were obtained and most of them belong to Basidiomycotina and Deuteromycotina. The preliminary biological activity test has shown that of these fungi 5 species help promote the seed germination of D.candidun , 7 species can establish the symbiotical relationship with seedlings of D.candium and D.nobile , and 3 species can stimulate the growth of seedlings of the two medicinal plants. Conclusion:Isolation and screening of fungi helpful to the growth and development of Dendrobium is important in the production of the herb.