| 广州地区呼吸道合胞病毒分离株G蛋白基因序列分析 |
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| 引用本文： | 阳隽,黄海鹭,徐军,钟南山.广州地区呼吸道合胞病毒分离株G蛋白基因序列分析[J].广东医学,2003,24(6):580-582. |
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| 作者姓名： | 阳隽 黄海鹭 徐军 钟南山 |
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| 作者单位： | 1. 广州医学院第二附属医院呼吸内科,广州,510260
2. 广东省广州呼吸疾病研究所,510120 |
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| 基金项目： | 国家自然科学基金资助项目 (编号 :39870 32 5) |
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| 摘 要： | 目的：探讨广州地区呼吸道合胞病毒(RSV)G蛋白基因变异的生物学意义，为研究广州地区BSV感染特点及RSV疫苗提供依据。方法：用PT—PCR的方法从广州地区呼吸道合胞病毒分离株98159株的细胞培养物中扩增出编码其表面蛋白G的基因片段，克隆至pGEM—T载体中并进行序列测定。结果：序列测定结果与A亚型原型株A2抹、1990年中国北京地区分离株B79G蛋白的核苷酸序列比较核苷酸同源率分别为92．7％及98．4％；由核苷酸序列推导的氨基酸序列的同源率分别为88.3％及97．0％。一些重要的免疫反应位点发生了变异。结论：RSVG蛋白抗原结构变异大，在研制疫苗时应注意选用株的地区性。
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| 关 键 词： | 广州地区 呼吸道合胞病毒 分离株 G蛋白 基因序列分析 |
| 修稿时间： | 2002年11月19 |
Sequence analysis of G protein gene of human respiratory syncytial virus in Guangzhou area |
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| Yang Jun,Huang Hailu,Xu Jun,et al..Sequence analysis of G protein gene of human respiratory syncytial virus in Guangzhou area[J].Guangdong Medical Journal,2003,24(6):580-582. |
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| Authors: | Yang Jun Huang Hailu Xu Jun |
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| Institution: | Yang Jun,Huang Hailu,Xu Jun,et al. Department of Respiratory Medicine,Second Affiliated Hospital,Guangzhou Medical College,Guangzhou 510260 |
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| Abstract: | Objective To study the sequence of G protein gene of human respiratory syncytial virus in Guangzhou area of China. Methods The cDNA encoding RSV G protein gene was amplified from HEP-2 cells infected RSV by RT-PCR and cloned into pGEM-T vector. The recombinant plasmid was sequenced. Results The nucleotide sequence for RSV G protein presented 92 7% and 98 4% overall nucleotide identity compared with those of RSV isolate A2 and B79 strain respectively. The deduced amino acid sequence of the cloned G protein presented with 88 3% and 97 0% overall amino acid identity compared with those of isolate A2 and B79 strain respectively. Variations of amino acid were found in some antigen epitopes. Conclusion Field RSV isolates may be optimal candidate for preparing vaccines in different areas. |
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| Keywords: | Respiratory syncytial virus G protein Nucleotide sequence |
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