| MCAb、PcAb不同标记ELISA技术对HBeAg／抗－HBe检测的评估 |
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| 引用本文： | 高志良,姚集鲁.MCAb、PcAb不同标记ELISA技术对HBeAg／抗－HBe检测的评估[J].中华实验和临床病毒学杂志,1994(3). |
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| 作者姓名： | 高志良 姚集鲁 |
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| 作者单位： | 中山医科大学附属第三医院传染科 |
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| 摘 要： | 用单克隆抗体（ＭｃＡｂ）和多克隆抗体（ＰｃＡｂ）进行辣根过氧化物酶标记或生物素标记作酶联免疫吸附试验（ＥＬＩＳＡ），检测乙型肝炎病毒ＨＢｅＡｇ／抗－ＨＢｅ标志物。结果显示，ＭｃＡｂ的灵敏性高于ＰｃＡｂ，且标记用的抗体量较少，一孔一期法同时测ＨＢｅＡｇ／抗－ＨＢｅ只能使用ＭｃＡｂ。利用生物素与亲和素系统与ＭｃＡｂ建立的Ｍｃ－ＡＢＣ－ＥＬＩＳＡ技术，其灵敏性与放射免疫法（ＲＩＡ）相同，特异性好，稳定性优于ＲＩＡ。生物素标记抗体至少１年保持效价不变，是一种新的较理想的检测ｅ系统的方法之一。
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| 关 键 词： | 生物素－亲和素系统，单克隆，多克隆抗－HBe抗体，酶联免疫吸附试验 |
A Comparison of Different ELISA Techniques Using Monoclonal or Polyclonal Antibody Reagents for the Detection of Antigen and Antibody |
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| Gao Zhiliang, Yao Jilu.A Comparison of Different ELISA Techniques Using Monoclonal or Polyclonal Antibody Reagents for the Detection of Antigen and Antibody[J].Chinese Journal of Experimental and Clinical Virology,1994(3). |
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| Authors: | Gao Zhiliang Yao Jilu |
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| Abstract: | Using monoclonal anti-HBe for labelling with enzyme or biotin, higher sensitivity was obtained and less antibody was needed when compared with using polyclonal antibody. The success of single well and one-step incubation technique for the detection of HBeAg and anti-HBe depended on using McAb rather than PcAb for coating solid phase. The Mc-ABC-ELISA technique was established and proved to be specific. Its sensitivity is similar to that of RIA. The tests of reproducibility showed that the anti-HBe conjugate was stable for at least one year. Therefore, Mc-ABC-ELISA is the method of choice for the detection of HBeAg and anti-HBe. |
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| Keywords: | Biotin-avidin system (BAS) Monoclonal or polyclonal anti-HBe ELISA |
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