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| 柯萨奇病毒B组3型基因疫苗预防实验性心肌炎的研究 | |
| 引用本文： | 田野,钟照华,杨巍,赵铁强,刘明,黄建林,孟繁超,黄永鳞,鲁凤民,叶鸿瑁. 柯萨奇病毒B组3型基因疫苗预防实验性心肌炎的研究[J]. 中国地方病学杂志, 2001, 20(4): 259-261 |
| 作者姓名： | 田野 钟照华 杨巍 赵铁强 刘明 黄建林 孟繁超 黄永鳞 鲁凤民 叶鸿瑁 |
| 作者单位： | 1. 哈尔滨医科大学第一临床医学院心内科 2. 哈尔滨医科大学基础医学院 3. 北京大学第三医院 |
| 基金项目： | 国家自然科学基金(39570744);黑龙江省自然科学基金 (D-9602) |
| 摘 要： | 目的：以构建的柯萨奇病毒B组3型（CV3）VP1基因的真核表达系统pCEP4-CVB3VP1作为基因疫苗，评价其对CVB3实验性心肌炎的预防作用。方法：大量制备pCEP4-CVB3VP1，提纯后将其接种BALB／C小鼠，取血清进行ELISA，病毒中和试验和病毒攻击保护实验，结果：ELISA证明pCEP4-CVB3VP1表达产物可刺激机体产生抗CVB3的的特异性IgM，病毒中和试验证明其可诱导机体产生中和抗体。中和CVB3毒力，病毒攻击保护实验证明其可保护接种小鼠抵抗CVB3攻击，结论：pCEP4-CVB3VP1能诱导机产生免疫应答，可作为基因疫苗预防CVB3心肌炎。 |
| 关 键 词： | 柯萨奇病毒 基因疫苗 实验性心肌炎 CVB3 VP1基因 |
| 文章编号： | 1000-4955(2001)04-0259-03 |
| 修稿时间： | 2000-10-31 |
A study on the effect of Coxsackievirus Group B Type 3 VP1 gene vaccine against experimental myocarditis |
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| TIAN Ye,ZHONG Zhao-hua,YANG Wei,et al. A study on the effect of Coxsackievirus Group B Type 3 VP1 gene vaccine against experimental myocarditis[J]. Chinese Jouranl of Endemiology, 2001, 20(4): 259-261 | |
| Authors: | TIAN Ye ZHONG Zhao-hua YANG Wei et al |
| Abstract: | Objective To evaluate the preventive effect of Coxsackievirus group B type 3 VP1 gene vaccine against experimental myocarditis.Methods pCEP4-CVB3VP1 was constructed by subcloning the VP1 gene into pCEP4. pCEP4-CVB3VP1 was large-scale prepared and inoculated BALB/C mice. Sera harvested from mice inoculated with pCEP4-CVB3VP1 were applied for ELISA and neutralization test. The cardiac pathology of inoculated mice attacked with CVB3 were examined.Results ELISA test showed the inoculated mice could produce CVB3 specific IgM and could neutralize the virulence of CVB3.Virus attack experiment showed the inoculated mice could resist the attack of CVB3.Conclusions pCEP4-CVB3VP1 may play as a gene vaccine to prevent viral myocarditis. |
| Keywords: | Coxsackievirus Gene vaccine Experimental myocarditis |
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