Application of liposomes to sonoporation |
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Authors: | Wu Junru Chen Di Pepe Jason Himberg Benjamin E Ricń Mercedes |
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Affiliation: | Department of Physics, University of Vermont, Burlington, VT 05405-0125, USA. JWU@ZOO.UVM.EDU |
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Abstract: | A method to prepare liposomes is presented. Liposomes made in our laboratory were characterized acoustically and optically. The phase velocity and attenuation of liposomes in suspension (concentration = 10(9)/mL) were measured, ranging from 2 to 14 MHz, using ultrasound spectroscopy. Anti-rabbit IgG conjugated with Alexafluor 647 was delivered into Jurkat cells in suspension, using the liposomes, by 10 % duty cycle ultrasound tonebursts of 2.2 MHz (the in situ spatial peak-pressure amplitude = 80 W/cm2) with an efficiency of 13 %. It has been experimentally shown that liposomes may be an alternative stable agent to Optison for delivering macromolecules into cells. |
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Keywords: | Ultrasound Liposomes Drug delivery In vitro cells |
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