丹参对大鼠肺缺血再灌注损伤保护作用的研究

目的：探讨丹参对大鼠肺缺血再灌注损伤的保护作用并探讨其机制。方法：60只wistar大鼠随机分为3组：手术对照组（A），缺血再灌注组（B），丹参干预组（C）。每组分别于缺血第45min、再灌注30、60、120min，观察肺组织病理形态变化，检测血浆丙二醛（MDA）、超氧化物歧化酶（SOD）的含量，测定肺组织湿／干重（W／D）比值，TUNEL法测定肺组织中细胞凋亡指数（AI）。结果：（1）肺组织病理变化：B组肺组织毛细血管充血、水肿、炎性细胞浸润显著，c组肺组织较B组减轻；（2）血浆MDA、SOD含量：经缺血再灌注后，B和C组血浆MDA较A组均明显增加（P〈0．05），B组增加的程度明显高于C组（P〈0．05）；B和C组血浆SOD较A组同时点均显著下降（P〈0．05），C组减少的程度明显小于B组（P〈0．05）；（3）肺组织W／D比值：经缺血再灌注后，B组和C组的肺组织W／D比值都呈进行性上升（再灌注60，120min vs缺血45min，P〈0．05）；C组上升幅度明显小于B组（再灌注60，120min时，C组vsB组，P〈0．05）；（4）肺组织细胞AI的变化：B组及C组缺血再灌注后均可见肺组织细胞凋亡现象，但与B组相比，C组相同时点的肺组织细胞凋亡数显著减少（P〈0．05）。结论：丹参对于实验性大鼠肺缺血再灌注损伤具有一定的保护作用，其作用机制与清除氧自由基、抗氧化及抗细胞凋亡有关。

A study of the protective effects of radix salviae miltiorrhizae on ischemia-reperfusion in-jury in rat lung

Objective:To study the mechanism of the protective effects of radix salvia miltiorrhiza on lung ischemia-reperfusion injury.Methods:Sixty Wistar rats were randomly divided into three groups:surgical control group(group A),ischemia-reperfusion group(group B)and Radix salvia miltiorrhiza group(group C).In each group,5 rats were sacrificed at one of four time points(45 min after ischemia;30 min,60 min and 120 min after reperfusion).The concentration of malondialdehyde(MDA)and superoxide dismutase(SOD)in plasma were detected,and the lung tissue wet/dry(W/D)ratio were examined.The histology of lung was observed,and the apoptotic index(AI)was detected by deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL).Results:(1).Histological changes:Marked pulmonary capillary congestion,edema and infiltration were observed in group B,while less pathologic alterations were shown in group C.(2).The concentration of MDA in plasma of both group B and C were significant higher at each time point after ischemia-reperfusion than that of group A(P<0.05).Between group B and C,the increased level of the former group was obviously higher than the latter one(P<0.05);The concentration of SOD in plasma of group B and C were significantly lower at each time point than that of group A(P<0.05),and the decreased level of group C was obviously lower than that of group B(P<0.05).(3)The W/D ratio of lung tissue in group B and C increased progressively with time after reperfusion(60 min,120 min reperfusion vs 45 min ischemia,P<0.05);but the increase degree of group C was obviously lower than that of group B(at 60 min,120 min after reperfusion,group C vs group B,P<0.05).(4).The AI of lung tissue were observed in both group B and group C,but AI in group C at different time points after reperfusion were all significant less than that in group B(P<0.05).Conclusion:radix salvia miltiorrhiza can effectively protect Wistar rats from ischemia-reperfusion lung injury and the mechanism is involved with its function of oxyradical elimination,antioxygen and anti-apoptosis.