白桦总三萜类成分高效液相色谱指纹图谱研究

目的建立白桦总三萜类成分的HPLC指纹图谱分析方法,研究不同采收期白桦药材的质量。方法以Kromasil C18柱（4.6mm×250mm,5μm）为色谱柱,流动相乙腈-水,采用梯度洗脱,流速为1.0ml/min,柱温25℃,检测波长280nm;进样量20μl;检测时间60min,对10批白桦总三萜类成分的HPLC指纹图谱进行研究,并运用相似度评价法对数据进行分析。结果实验标定9个共有峰,不同采收期的白桦指纹图谱相似度较好。结论该方法准确可靠,重复性好,用于白桦的质量评价切实可行;不同采收期白桦总三萜类化学组成相似,其相对比例较稳定。

High performance liquid chromatographic analysis of fingerprint of triterpenens of betula platphylla

ObjectiveTo establish HPLC fingerprint of total triterpenes of Betula Plantyphylla in order to control the quality of Betula Plantyphylla in different collection seasons.Methods HPLC method was performed on a Kromasil C18 column（4.6mm×250mm,5μm） with acetonitrile-water as mobile phase by gradient elution,the flow rate was at 1.0ml/min,column temperature was 25℃,detection wavelength was set at 280nm,sample size was 20μl and eluting period was 60min.Furthermore,similarity evaluation was used for data analysis.Results The experiment showed 9 shared peaks.Samples from different collection seasons were of high similarity.Conclusions This method with good accuracy and specificity can be used for quality control of Betula platyphylla;and the chemical compositions of total triterpenes from different harvest seasons are similar and stable.