| 口蹄疫病毒融合表位基因在pET原核系统的高表达及产物的纯化 |
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| 引用本文： | 陈祖欢,张洪英,郭瀛军,林懿,朱维佳,孙树汉. 口蹄疫病毒融合表位基因在pET原核系统的高表达及产物的纯化[J]. 第二军医大学学报, 2003, 24(8): 904-905 |
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| 作者姓名： | 陈祖欢 张洪英 郭瀛军 林懿 朱维佳 孙树汉 |
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| 作者单位： | 第二军医大学基础医学部医学遗传学教研室,上海,200433 |
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| 基金项目： | 国家“8 63”计划资助项目 ( 2 0 0 1AA2 13 111) |
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| 摘 要： | 构建口蹄疫融合表位基因-SG表达载体pET-SG,转化重组子与K802,新鲜过夜菌以2％接种量接种2YT培养液,37℃培养2 h后用IPTG诱导,每隔1 h取样,共8 h.根据SDS-PAGE结果,口蹄疫融合表位基因-SG在pET原核表达系统可获得较高表达,用IPTG诱导4 h蛋白表达量最高,用Ni+柱亲和层析可得到较纯蛋白.
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| 关 键 词： | 口蹄疫病毒、表位、基因表达、pET原核系统 |
| 文章编号： | 0258-879X(2003)08-0904-02 |
| 修稿时间： | 2003-01-15 |
High expression of fusion epitopes cDNA of foot-and-mouth disease virus in pET proeukaryotic system and purification of products |
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| CHEN Zu Huan,ZHANG Hong Ying,GUO Ying Jun,LIN Yi,ZHU Wei Jia,SUN Shu Han. High expression of fusion epitopes cDNA of foot-and-mouth disease virus in pET proeukaryotic system and purification of products[J]. Former Academic Journal of Second Military Medical University, 2003, 24(8): 904-905 |
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| Authors: | CHEN Zu Huan ZHANG Hong Ying GUO Ying Jun LIN Yi ZHU Wei Jia SUN Shu Han |
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| Affiliation: | CHEN Zu Huan,ZHANG Hong Ying,GUO Ying Jun,LIN Yi,ZHU Wei Jia,SUN Shu Han * |
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| Abstract: | Proeukaryotic expression vector pET SG for fusion epitopes of foot and mouth disease virus(FMDV) was constructed and transferred into K802.Fresh bacterium was added to 2YT culture medium at a radio of 2% and the system was incubated for 2 h and then induced by IPTG.Expression products was collected every hour and the best expression time was decided by SDS PAGE.FMDV epitope genes SG was expressed with high yield in pET proeukaryotic system,K802 pET SG produced the largest amout of fusion protein when induced by IPTG for 4 h, and pure fusion proteins was obtained through a Ni + affinity chromatography column. |
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| Keywords: | foot and mouth disease virus epitopes gene expression pET proeukaryotic system |
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