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| 高效液相色谱法同时测定甘草中甘草酸、甘草苷、异甘草素的含量 | |
| 引用本文： | 陈云华,赵晓霞,王文全,段天璇.高效液相色谱法同时测定甘草中甘草酸、甘草苷、异甘草素的含量[J].中国中医药信息杂志,2009,16(8):52-54. |
| 作者姓名： | 陈云华 赵晓霞 王文全 段天璇 |
| 作者单位： | 1. 北京城市学院生物技术学部,北京,100094 2. 北京因科瑞斯生物制品研究所,北京,102209 3. 北京中医药大学中药学院,北京,100102 |
| 摘 要： | 目的建立同时测定甘草中甘草酸、甘草苷和异甘草素含量的方法。方法采用高效液相色谱法对甘草粉末的67％甲醇提取物进行分析。用C18反相色谱柱，1％磷酸水-乙腈梯度洗脱，对不同色谱峰分别采用248、276、360、370nm紫外波长检测。结果甘草酸的回归方程为，=1×10^6X＋16220，r^2=1；甘草苷的回归方程为r=2×10^6X＋49444，r^2=0．9995；异甘草素的回归方程为r=1×10^7X＋4．4667，r^2=1。甘草酸、甘草苷和异甘草素的加样回收率分别为98．01％、102．63％、98．18％。结论本方法准确、稳定、可靠，可用于甘草中甘草酸、甘草苷和异甘草素3种成分的同时测定。 |
| 关 键 词： | 甘草 甘草酸 甘草苷 异甘草素 高效液相色谱法 含量测定 |
Simultaneous Determination of Glycyrrhizin,Liquiritin and Isoliquritigenin in Licorice by HPLC |
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| Institution: | CHEN Yun-hua, ZHAO Xiao-xia, WANG Wen-quan, et al (1.Biotechnology College, Beijing City University, Beijing 100094, China： 2.Beo＇ing lncrease Biological Product Institute, Beo＇ing 102209, China： 3.Pharmacy College, Beijing University ofTCM, Beijing 100102, China) |
| Abstract: | Objective To develop a HPLC quantitatively test method of glycyrrhizin, liquiritin and isoliquritigenin simultaneously for licorice. Methods The 67% methanol extracts of licorice powder were analyzed by reverse-phase HPLC on an ODS column, using a gradient elution with a mixture of 1% H3PO4 and acetonitrile. The eluate was monitored by UV absorption at 248, 276, 360 and 370 nm. Results The linear equation of glycyrrhizin was Y = 1 × 10^6X ＋ 16 220, r^2 = 1. The linear equation of liquiritin was Y = 2 ×10^6X ＋ 49 444, r^2 = 0.999 5. The linear equation of isoliquritigenin was Y = 1 × 10^7X ＋ 4.466 7, r ^2 = 1. The recovery rate of glycyrrhizin, liquiritin and isoliquritigenin was 98.01%, 102.63% and 98.18% respectively. Conclusion The method is accurate, stable amd reliable, and can be used to determine the three main chemical contents simultaneously. |
| Keywords: | Licorice glycyrrhizin liquiritin isoliquritigenin HPLC content determination |
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