Ad-p53诱导乳腺癌细胞MDA-MB-231的凋亡作用

目的：观察重组人p53腺病毒（ p53-expressing adenovirus，Ad-p53）对人乳腺癌细胞MDA-MB-231细胞生长、细胞周期、凋亡和p53表达的影响。方法：流式细胞仪检测Ad-p53作用于MDA-MB-231细胞72h后细胞凋亡率和细胞周期变化，Western blot法检测MDA-MB-231细胞p53蛋白表达。结果：经Ad-p53处理72h后倒置显微镜下MDA-MB-231细胞形态发生明显的变化。流式细胞术检测结果显示， Ad-p53作用MDA-MB-231后其细胞凋亡率与对照组相比明显增加，G0/G1期细胞比例逐渐升高，S期、G2/M期细胞比例相应减少（ P＜0.05）。Western blot证实了外源野生型p53基因能在MDA-MB-231细胞表达增加。结论：Ad-p53可以抑制人乳腺癌细胞株MDA-MB-231的生长，促进其凋亡，其机制可能是通过阻滞细胞周期以及野生型p53蛋白表达增加实现的。

Effect of Ad-p53 on induction of apoptosis in MDA-MB-231 breast cancer cells

Objective：To investigate the effect of Ad-p53（ p53 -expressing adenovirus）on the expression of p53 gene and the growth,cell cycle and apoptosis of MDA-MB-231 cells. Methods：The changes of human breast cancer cell line MDA-MB-231 morphology were observed by inverted microscopy. The apoptosis rate and cell cycle were analyzed by flow cytometry. Western blot was used to detect the protein expression of p53. Results：Significant changes of cell morphology in MDA-MB-231cells were observed by an inverted microscope. Flow cytometry re-vealed that Ad-p53 could increase the cell proliferation inhibition rate,and there was significant difference between MDA-MB-231 group and control group,and the proportion of MDA-MB-231 cells in S phase and G2/M phase decreased but increased in G0/G1 phase. Western blot results indicated that the p53 protein expression level in MDA-MB-231 cells increased obviously with Ad-p53. Conclusion：Ad-p53 can inhibit the cell growth and induce the cell apoptosis,which may be related to inhibition of the cell cycle and increase in the expression of wild-type p53 protein.