| 重组人血管内皮抑素对肺腺癌A549细胞生长及凋亡的影响 |
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| 引用本文： | 夏荣耀,;张冉冉,;陈复辉.重组人血管内皮抑素对肺腺癌A549细胞生长及凋亡的影响[J].现代肿瘤医学,2014(7):1511-1514. |
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| 作者姓名： | 夏荣耀 ;张冉冉 ;陈复辉 |
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| 作者单位： | [1]哈尔滨医科大学附属第二医院呼吸内科,黑龙江哈尔滨150086; [2]哈尔滨医科大学附属第二医院肿瘤内科,黑龙江哈尔滨150086 |
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| 基金项目： | 黑龙江省自然基金（编号：D201079） |
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| 摘 要： | 目的：探讨重组人血管内皮抑素（恩度）对肺腺癌A549细胞生长及凋亡的影响。方法：应用四甲基偶氮唑蓝（MTT）法检测恩度对肺腺癌A549细胞的生长抑制作用，流式细胞仪检测肺腺癌A549细胞的凋亡率，比较各浓度药物对肺腺癌A549细胞的诱导凋亡作用。结果：恩度可以抑制肺腺癌A549细胞的生长，且呈时间剂量依赖性。恩度可以诱导肺腺癌A549细胞的凋亡，随浓度增加、作用时间的延长，凋亡率增加，与对照组相比，差异有统计学意义（P〈0．001）。结论：恩度对肺腺癌A549细胞的生长具有明显的抑制作用，主要通过诱导肺腺癌A549细胞的凋亡引起。
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| 关 键 词： | 恩度 肺腺癌A549细胞 生长抑制 细胞凋亡 |
Effect of endostar on the growth and apoptosis of lung cancer A549 cells |
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| Institution: | Xia Rongyao ,Zhang Ranran, Chen Fuhui(1 Department of Respiratory; 2 Department of Medical Oncology, The Second Affiliated Hospital of Harbin Medical University, Heilongjiang Harbin 150086, China.) |
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| Abstract: | Objective：To investigate the effect of endostatin on A549 lung cancer. Methods：MTT was used to de tect the inhibitory effect of A549 lung cancer cells treated with endostar at the concentration of 50,100,200,400μg/ml for 24h,48h or 72h. TUNEL assay was used to detect apoptosis rate of As49 lung cancer ceils treated with endostar at the concentration of 50,100,200,4001xg/ml for 24h or 48h. Results ： All concentrations of endostatin can inhibit the proliferation of As49 lung cancer ceils, and the inhibitory effect increased with the concentration. Endostar induced ap optosis of A549 lung cancer cells, and it was dose and time dependent. Conclusion： Endostatin inhibited the pro liferation and induced apoptosis of A549 lung cancer cells,it was dose and time dependent. |
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| Keywords: | endostar As49 lung cancer cells inhibition apoptosis |
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